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首页> 外文期刊>Journal of Theoretical Biology >Determination of kinetic parameters of enzyme-catalyzed reactions with a minimum number of velocity measurements.
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Determination of kinetic parameters of enzyme-catalyzed reactions with a minimum number of velocity measurements.

机译：用最少的速度测量来确定酶催化反应的动力学参数。

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摘要

Duggleby [Duggleby, R.G., 1979. Experimental designs for estimating kinetic parameters for enzyme-catalyzed reactions. J. Theor. Biol. 81, 672-684] discussed the "design of several replicate measurements of the velocity at as many experimental conditions as there are parameters to be estimated." He discussed the application of this method to A-->products, without and with competitive inhibition, and commented briefly on A+B-->products. The availability of computer applications that can solve large sets of simultaneous equations makes it possible to use this method to calculate kinetic parameters for more complicated enzyme mechanisms. This article is concerned with rapid-equilibrium rate equations, but this method can also be used with steady-state rate equations. Computer programs are provided for the calculation of the three kinetic parameters for ordered A+B-->products from three velocity measurements and for the calculation of the four kinetic parameters for random A+B-->products from four velocitymeasurements. Computer programs are also provided for competitive inhibition, uncompetitive inhibition, and mixed inhibition of ordered A+B-->products.
机译：Duggleby [Duggleby，R.G.，1979年。用于估算酶催化反应动力学参数的实验设计。 J.理论。生物学[81，672-684]讨论了“在与要估计的参数一样多的实验条件下对速度进行多次重复测量的设计”。他讨论了在没有竞争抑制和有竞争抑制的情况下将该方法应用于A->产品的方法，并简要评论了A + B->产品。可以解决大量联立方程组的计算机应用程序的可用性使得可以使用此方法来计算动力学参数以用于更复杂的酶机制。本文关注快速平衡速率方程，但该方法也可用于稳态速率方程。提供了计算机程序，用于从三个速度测量中计算有序A + B->乘积的三个动力学参数，并从四个速度测量中计算随机A + B->乘积的四个动力学参数。还提供了用于竞争性抑制，非竞争性抑制和有序A + B->产品混合抑制的计算机程序。

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