...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Large-scale expansion of pre-isolated bone marrow mesenchymal stromal cells in serum-free conditions
【24h】

Large-scale expansion of pre-isolated bone marrow mesenchymal stromal cells in serum-free conditions

机译：无血清条件下预分离的骨髓间充质基质细胞的大规模扩增

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The regenerative potential of mesenchymal stromal or stem cells (MSCs) has generated tremendous interest for treating various degenerative diseases. Regulatory preference is to use a culture medium that is devoid of bovine components for stem cell expansion intended for therapeutic applications. However, a clear choice an alternative to fetal bovine serum (FBS) has not yet emerged. We have screened five different commercially available serum-free media (SFM) for their ability to support the growth and expansion of pre-isolated undifferentiated bone marrow-derived MSCs (BM-MSCs) and compared the results with cells grown in standard FBS-containing medium as control. In addition, based on initial screening results, BD Mosaic Mesenchymal Stem Cell Serum-free (BD-SFM) medium was evaluated in large-scale cultures for the performance and culture characteristics of BM-MSCs. Of the five different serum-free media, BD-SFM enhanced BM-MSCs growth and expansion in Cell STACK (CS), but the cell yield per CS-10 was less when compared to the control medium. The characteristics of MSCs were measured in terms of population doubling time (PDT), cell yield and expression of MSC-specific markers. Significant differences were observed between BD-SFM and control medium in terms of population doublings (PDs), cell yield, CFU-F and morphological features, whereas surface phenotype and differentiation potentials were comparable. The BD-SFM-cultured MSCs were also found to retain the differentiation potential, immune-privileged status and immunosuppressive properties inherent to MSCs. Our results suggest that BD-SFM supports large-scale expansion of BM-MSCs for therapeutic use. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：间充质基质或干细胞（MSCs）的再生潜力已引起人们对治疗各种退行性疾病的巨大兴趣。监管上的优先选择是使用不含牛成分的培养基进行干细胞扩增，以用于治疗应用。但是，尚未出现替代胎牛血清（FBS）的明确选择。我们已经筛选了五种不同的市售无血清培养基（SFM），它们具有支持预分离的未分化骨髓来源MSC（BM-MSC）生长和扩展的能力，并将结果与​​在含FBS的标准细胞中生长的细胞进行了比较介质作为对照。此外，基于初步筛选结果，在大规模培养中评估了BD马赛克间充质干细胞无血清（BD-SFM）培养基的BM-MSC的性能和培养特性。在五种不同的无血清培养基中，BD-SFM增强了BM-MSC在Cell STACK（CS）中的生长和扩增，但与对照培养基相比，每CS-10的细胞产量要低。通过群体倍增时间（PDT），细胞产量和MSC特异性标志物的表达来测量MSC的特征。在种群倍增（PD），细胞产量，CFU-F和形态特征方面，BD-SFM与对照培养基之间存在显着差异，而表面表型和分化潜能却相当。还发现，BD-SFM培养的MSC保留了MSC固有的分化潜能，免疫特权状态和免疫抑制特性。我们的结果表明，BD-SFM支持用于治疗用途的BM-MSC的大规模扩展。版权所有（c）2014 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号