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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >The effect of fresh bone marrow cells on reconstruction of mouse calvarial defect combined with calvarial osteoprogenitor cells and collagen-apatite scaffold
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The effect of fresh bone marrow cells on reconstruction of mouse calvarial defect combined with calvarial osteoprogenitor cells and collagen-apatite scaffold

机译：新鲜骨髓细胞联合颅骨造血祖细胞和胶原-磷灰石支架对小鼠颅骨缺损的修复作用

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摘要

Fresh bone marrow cells have already exhibited its advantages as osteogenic donor cells, but the combination between fresh bone marrow cells and other donor cells utilized for bone healing has not been fully explored. To highlight the impact of fresh bone marrow cells on scaffold-based bone regeneration, single or a combination of calvarial osteoprogenitor cells (OPCs) and bone marrow cells (BMCs) were used as donor cells combined with collagen-apatite scaffold for calvarial defect healing. The host and donor contributions to bone formation were assessed using histological and GFP imaging analysis. Although the amount of new bone formed by different cell sources did not show significant differences, the origin of the bone formation in the defects mainly depended on the types of donor cells employed: when only calvarial OPCs were used as donor cells, a donor-derived bone healing instead of host-derived bone ingrowth was observed; when only fresh BMCs were loaded, the host bone could grow into the defect along the lamellar structure of the scaffolds, but the amount of new bone formed was significantly lower than the defect loaded with calvarial OPCs only. The combination of calvarial OPCs and fresh BMCs had similar amount of new bone formation as the group loaded with calvarial osteoprogenitors alone, but did not induce any host-derived bone formation. These results provide compelling evidence of the importance of fresh BMCs to induce host-implant integration in bone tissue engineering.
机译：新鲜的骨髓细胞已经显示出其作为成骨性供体细胞的优势，但是尚未充分探索新鲜的骨髓细胞与其他用于骨愈合的供体细胞之间的组合。为了强调新鲜骨髓细胞对基于支架的骨再生的影响，将颅盖骨祖细胞（OPC）和骨髓细胞（BMC）的单个或组合用作供体细胞，并结合胶原磷灰石支架来修复颅骨缺损。使用组织学和GFP成像分析评估宿主和供体对骨形成的贡献。尽管不同细胞来源形成的新骨量没有显着差异，但缺陷中骨形成的来源主要取决于所用供体细胞的类型：当仅使用颅盖骨干细胞作为供体细胞时，源自供体观察到骨愈合而不是宿主来源的骨向内生长;当仅加载新鲜的BMC时，宿主骨骼可以沿着支架的层状结构生长到缺损中，但是形成的新骨骼的数量明显低于仅使用颅骨OPC加载的缺损。颅骨OPC和新鲜BMC的组合具有与单独颅骨骨祖细胞组相似的新骨形成量，但不会诱导任何宿主来源的骨形成。这些结果提供了令人信服的证据，表明新鲜的BMC在骨组织工程中诱导宿主-植入物整合的重要性。

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