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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Evaluation of a novel collagen-gelatin scaffold for achieving the sustained release of basic fibroblast growth factor in a diabetic mouse model
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Evaluation of a novel collagen-gelatin scaffold for achieving the sustained release of basic fibroblast growth factor in a diabetic mouse model

机译：评价新型胶原蛋白-明胶支架在糖尿病小鼠模型中实现碱性成纤维细胞生长因子的持续释放

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The objective of this study was to evaluate the ability of a scaffold, collagen-gelatin sponge (CGS), to release basic fibroblast growth factor (bFGF) in a sustained manner, using a pressure-induced decubitus ulcer model involving genetically diabetic mice. We confirmed that CGSs impregnated with a bFGF concentration of up to 50μg/cm2 were able to sustain the release of bFGF throughout their biodegradation. We prepared decubitus ulcers on diabetic mice. After debriding the ulcers, we implanted CGSs (diameter 8mm) impregnated with normal saline solution (NSS) or bFGF solution (7, 14, 28 or 50μg/cm2). At 1 and 2weeks after implantation, the mice were sacrificed and tissue specimens were obtained. The wound area, neoepithelium length and numbers and total area of newly formed capillaries were evaluated. The CGSs impregnated with NSS became infected and degraded, whereas the CGSs impregnated with 7 or 14μg/cm2 bFGF displayed accelerated dermis-like tissue formation and the CGSs impregnated with 14μg/cm2 bFGF produced significant improvements in the remaining wound area, neoepithelium length and numbers and total area of newly formed capillaries compared with the NSS group. No significant difference was observed between the NSS and 50μg/cm2 bFGF groups. CGSs impregnated with 7-14μg/cm2 bFGF accelerated wound healing, and an excess amount of bFGF did not increase the wound-healing efficacy of the CGSs. Our CGS is a scaffold that can release positively charged growth factors such as bFGF in a sustained manner and shows promise as a scaffold for skin regeneration.
机译：这项研究的目的是使用涉及遗传性糖尿病小鼠的压力诱发的褥疮溃疡模型，评估支架胶原明胶海绵（CGS）持续释放碱性成纤维细胞生长因子（bFGF）的能力。我们证实，浸渍了bFGF浓度高达50μg/ cm2的CGS能够在整个生物降解过程中维持bFGF的释放。我们在糖尿病小鼠上准备了褥疮性溃疡。清除溃疡后，我们植入CGS（直径8毫米），并用生理盐溶液（NSS）或bFGF溶液（7、14、28或50μg/ cm2）浸渍。植入后1和2周，处死小鼠并获得组织标本。评价伤口面积，新上皮长度，新形成的毛细血管的数目和总面积。浸渍有NSS的CGS被感染并降解，而浸渍有7或14μg/ cm2 bFGF的CGS表现出加速的真皮样组织形成，而浸渍有14μg/ cm2 bFGF的CGS则在剩余伤口面积，新上皮长度和数目方面产生了显着改善。和新形成的毛细血管的总面积（与NSS组相比）。 NSS组和50μg/ cm2 bFGF组之间未观察到显着差异。用7-14μg/ cm2 bFGF浸渍的CGS可以促进伤口愈合，而过量的bFGF不能提高CGS的伤口愈合功效。我们的CGS是一种可以持续释放带正电荷的生长因子（例如bFGF）的支架，并有望作为皮肤再生的支架。

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