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首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >The interlobular distribution of copper in the liver of beef calves on a high-copper diet.
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The interlobular distribution of copper in the liver of beef calves on a high-copper diet.

机译:高铜饮食下牛犊肝脏中铜的小叶间分布。

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摘要

The aims of the present study were (1) to evaluate the interlobular distribution of copper (Cu) in the liver of beef calves on a high-Cu diet, (2) to determine whether this distribution differs between Galician Blonds and Holstein Friesians, and (3) to determine whether in vivo needle biopsy provides an appropriate measure of overall hepatic Cu status. Liver biopsies were performed before slaughter on twenty-nine 10-month-old beef calves fed growing and finishing diets supplemented with 35 mg/kg of Cu sulfate (10 Galician Blonds, 9 Holstein Friesians, and 10 Galician Blond x Holstein Friesian crosses). At slaughter, samples taken from 6 regions of the liver (the internal and external faces of the right lobe; the left, caudate, and quadrate lobes; and the processus papillaris) were acid digested, and their Cu contents were determined by inductively coupled plasma atomic emission spectrometry. The highest Cu concentrations were found in the left lobe, followed by the processus papillaris, and the lowest Cu concentrations were found in the caudate and quadrate lobes. Different breeds differ in absolute hepatic Cu levels, but interlobular Cu distribution does not appear to depend on breed, at least when Galician Blonds are compared with Holstein Friesians. In vivo needle biopsy afforded accurate estimates of overall hepatic Cu status.
机译:本研究的目的是(1)评估高铜饮食的牛犊肝脏中铜(Cu)的小叶间分布;(2)确定这种分布在加利西亚金发和荷尔斯泰因弗里斯兰人之间是否有所不同,以及(3)确定体内针头活检是否提供了总体肝Cu状态的适当测量。屠宰前,对生长和日粮添加35 mg / kg硫酸铜的10个月大的小牛进行屠宰(10加利西亚金发,9荷斯坦黑白花和10加利西亚金发x荷斯坦黑白花杂交)。屠宰时,酸消化肝的六个区域(右叶的内表面和外表面;左,尾状和方形叶;乳突)的样品,并通过电感耦合血浆测定其铜含量。原子发射光谱法。在左叶中发现最高的铜浓度,其次是乳突突,而在尾状和方形叶中发现的铜浓度最低。不同品种的绝对肝铜水平不同,但是至少在加利西亚金发女郎和荷斯坦弗里斯兰人比较时,小叶间铜的分布似乎并不取决于品种。体内穿刺活检可准确估算总体肝铜状态。

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