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首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats.
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Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats.

机译：蝙蝠白鼻综合症的快速聚合酶链反应诊断。

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摘要

A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals.
机译：本研究描述了一种基于聚合酶链反应（PCR）的新方法，该方法可从少量（1-2 mg）组织中快速特异性地检测被感染蝙蝠翅膀上的 Geomyces destructans 。还描述了从蝙蝠皮中培养和分离 G。destructans 的方法。 PCR检测的下限是每2 mg机翼组织5 fg纯化真菌DNA或100分生孢子。以组织学为标准，PCR的诊断特异性为100％，诊断灵敏度为96％，而培养技术的诊断灵敏度仅为54％。 PCR的准确性和快速的周转时间比传统方法为野外生物学家提供了有关感染状况的有价值的信息，而且该测试所需的少量组织可用于诊断活体动物的白鼻综合症。

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