• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of travel medicine. >Enterotoxigenic Escherichia coli and diffusely adherent E. coli as likely causes of a proportion of pathogen-negative travelers' diarrhea--a PCR-based study.
【24h】

Enterotoxigenic Escherichia coli and diffusely adherent E. coli as likely causes of a proportion of pathogen-negative travelers' diarrhea--a PCR-based study.

机译：肠毒素大肠杆菌和弥漫性大肠杆菌可能是造成一定比例的病原体阴性旅行者腹泻的一项基于PCR的研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Enteropathogens cannot be identified in 40% to 50% of subjects with travelers' diarrhea (TD). METHODS: We used polymerase chain reaction (PCR) methods to look for the presence of two bacterial causes of diarrhea in a large group of international travelers after failing to detect a pathogen by conventional tests. DNA was isolated from the diarrheal stool and subjected to PCR from 162 subjects from whom we earlier failed to identify a pathogen in a previous study and included 54 from Antigua, Guatemala, 39 from Guadalajara, Mexico, 29 from Kolkata, India, and 40 from Goa, India. Gene products for enterotoxigenic Escherichia coli (ETEC)--LT (heat-labile enterotoxin) and ST (heat-stable enterotoxin)--and diffusely adherent E. coli (DAEC), afa/dr (Afa fimbrial and Dr nonfimbrial family of adhesins), were used. RESULTS: At least one gene product was identified in diarrhea stool samples of 47 of 162 (29%) subjects. ETEC virulence genes (LT, ST) were found in 34 (21%) samples studied, with rates of occurrence ranging from 8% in Goa to 39% for the samples from Guatemala (p = 0.0006). A large number of ST-only strains explained the high ETEC rate in Guatemala. DAEC afa/dr family of adhesions was identified in between 8 and 14% of the samples. CONCLUSIONS: ETEC and DAEC were implicated in nearly one-third of the subjects initially diagnosed as pathogen negative. Direct PCR results from stools are consistent with the previous assumption that most undiagnosed TD is bacterial in nature and also highlights the potential value that PCR can add to studies designed to evaluate treatment and preventive interventions for TD, including vaccines.
机译：背景：在40％至50％的旅行者腹泻（TD）患者中无法鉴定出肠病原体。方法：我们使用聚合酶链反应（PCR）方法在大批国际旅行者中通过常规测试未能检测到病原体后，寻找导致细菌性腹泻的两种细菌。从腹泻粪便中分离出DNA，并对其进行PCR扩增，我们先前在先前的研究中未能从中鉴定出162名受试者，其中包括危地马拉安提瓜的54名，墨西哥瓜达拉哈拉的39名，印度加尔各答的29名和印度的40名。印度果阿。产肠毒素大肠埃希菌（ETEC）-LT（不耐热肠毒素）和ST（热稳定肠毒素）-以及弥散性大肠杆菌（DAEC），afa / dr（Afa纤维和Dr非纤维家族粘附蛋白家族）的基因产物）。结果：在162名（29％）受试者的47名腹泻粪便样本中至少鉴定出一种基因产物。在研究的34个样本中发现了ETEC毒力基因（LT，ST）（21％），果阿的发生率从8％到危地马拉的39％（p = 0.0006）。大量仅ST菌株解释了危地马拉的ETEC率很高。在8％至14％的样品中发现了DAEC afa / dr粘连家族。结论：ETEC和DAEC与最初诊断为病原体阴性的受试者近三分之一有关。粪便的直接PCR结果与先前的假设一致，即大多数未诊断的TD本质上是细菌，并且还突出了PCR可以为旨在评估TD（包括疫苗）的治疗和预防干预措施的研究增加潜在价值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号