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首页> 外文期刊>Clinical oral investigations >Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis
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Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis

机译：中国成年人慢性牙周炎唾液，龈上和龈下菌斑中微生物群的定量分析

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Objective: The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR). Materials and methods: Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia as well as total bacterial counts from 252 samples collected from the saliva, supragingival plaque, and subgingival plaque of all 84 subjects. Results: The detection frequency of A. actinomycetemcomitans was less than 50%. F. nucleatum was detected in all subjects and CP patients had higher bacterial loads than healthy subjects. The median proportion of F. nucleatum was significantly higher in subgingival plaque than in supragingival plaque and saliva. P. gingivalis and P. intermedia had higher detection frequencies and bacterial loads in CP patients than in healthy subjects. The median proportion of P. gingivalis was significantly different among the three intraoral locations in the CP group and its proportion in subgingival plaque was 9. 01%. Moreover, strong positive Spearman's correlations were found in A. actinomycetemcomitans, P. gingivalis, and P. intermedia counts across the three intraoral locations. Conclusion: The presence and bacteria loads of these four bacteria in this Chinese population are similar to those from other populations. Clinical relevance: Examination of bacterial detection frequency and loads in Chinese adults may assist microbial studies of periodontal disease and will shed light on periodontal disease diagnosis and treatment using antibiotics in the Chinese population.
机译：目的：本研究的目的是使用定量实时聚合酶链反应（qRT-PCR）确定中国人群中牙周致病菌的概况。材料和方法：本研究纳入了二十四名中国牙周健康受试者和60名慢性牙周炎（CP）患者。使用qRT-PCR定量分析所有84位受试者的唾液，龈上菌斑和龈下菌斑的252个样本中的细菌总数，包括252个样品的唾液，龈上菌斑和龈下菌斑，并对放线杆菌，核纤梭菌，牙龈卟啉单胞菌和中间细菌进行定量。结果：A。放线菌的检出频率小于50％。在所有受试者中均检测到了F. nucleatum，CP患者的细菌载量高于健康受试者。龈下斑块中的核仁镰刀菌的中值比例显着高于龈上斑块和唾液。与健康受试者相比，CP患者的齿龈假单胞菌和中间媒介假单胞菌具有更高的检测频率和细菌载量。 CP组的三个口内位置之间牙龈卟啉单胞菌的中位比例显着不同，在牙龈下菌斑中的比例为9. 01％。此外，在三个口腔内位置的放线菌，齿龈假单胞菌和中间假单胞菌计数中发现了强烈的正Spearman相关性。结论：这4种细菌在中国人群中的存在和细菌载量与其他人群相似。临床意义：在中国成年人中检查细菌的检出频率和负荷可能有助于牙周疾病的微生物研究，并为在中国人群中使用抗生素进行牙周疾病的诊断和治疗提供启示。

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