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首页> 外文期刊>Journal of trace elements in medicine and biology: Organ of the Society for Minerals and Trace Elements (GMS) >Genotoxicity of cadmium chloride in human lymphocytes evaluated by the comet assay and cytogenetic tests.
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Genotoxicity of cadmium chloride in human lymphocytes evaluated by the comet assay and cytogenetic tests.

机译：彗星试验和细胞遗传学试验评估了氯化镉对人淋巴细胞的遗传毒性。

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摘要

Peripheral blood lymphocytes were tested in vitro for genotoxic effects of cadmium chloride. Whole blood samples of four healthy, non-smoking subjects were preincubated with CdCl2 in concentrations of 10(-4), 10(-3), and 5 . 10(-3) mol/L for three hours before the cells were assessed for DNA-damage using the single cell alkaline gel electrophoresis assay (comet assay) or cultivated for chromosomal aberrations (CA), sister chromatid exchanges (SCE), and the micronucleus (MN) test. The comet assay showed notable interindividual differences. The results of the cytogenetic tests showed an increase in the frequency of CA, MN, and SCE with CdCl2 in the treated cultures, yet none was able to show a correlation between concentrations of cadmium chloride and the frequency of damages. The MN slides were stained with Giemsa and with DNA fluorochrome 4', 6'-diamidino-2-phenylindole (DAPI). The frequency of MN in slides stained with DAPI was significantly higher than in those stained with Giemsa, which might be due to an underestimation of small micronuclei in Giemsa-stained slides.
机译：在体外测试了外周血淋巴细胞对氯化镉的遗传毒性作用。将四名健康的非吸烟受试者的全血样品与CdCl2分别以10（-4），10（-3）和5的浓度孵育。 10（-3）mol / L持续三小时，然后使用单细胞碱性凝胶电泳测定法（彗星测定法）评估细胞的DNA损伤，或针对染色体畸变（CA），姊妹染色单体交换（SCE）和微核（MN）测试。彗星试验显示出明显的个体差异。细胞遗传学测试的结果表明，在处理过的培养物中，使用CdCl2的CA，MN和SCE的频率增加，但是没有一个能够显示出氯化镉浓度与损害频率之间的相关性。 MN玻片用Giemsa和DNA荧光染料4'，6'-二mid基-2-苯基吲哚（DAPI）染色。用DAPI染色的载玻片中MN的频率显着高于用Giemsa染色的载玻片，这可能是由于低估了Giemsa染色的载玻片中的小微核。

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