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首页> 外文期刊>Communications in Agricultural and Applied Biological Sciences >POTENTIALITY OF USING MICROBIAL BIOSENSORS FOR THE DETECTION OF SUBSTRATE HETEROGENEITIES AND THE ASSESSMENT OF MICROBIAL VIABILITY IN INDUSTRIAL BIOREACTORS: A COMPLETE SET OF EXPERIMENTS IN CHEMOSTAT AND SCALE DOWN REACTORS, AND ELABORATION OF A MINI SCALE-DOWN PLATFORM
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POTENTIALITY OF USING MICROBIAL BIOSENSORS FOR THE DETECTION OF SUBSTRATE HETEROGENEITIES AND THE ASSESSMENT OF MICROBIAL VIABILITY IN INDUSTRIAL BIOREACTORS: A COMPLETE SET OF EXPERIMENTS IN CHEMOSTAT AND SCALE DOWN REACTORS, AND ELABORATION OF A MINI SCALE-DOWN PLATFORM

机译：利用微生物传感器检测基质异质性和工业生物反应器中微生物活性评估的潜力：一套完整的化学计量器和规模缩减反应器实验，以及一个小型规模化平台的建立

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摘要

Substrate limitation responsive biosensors have been used in order to detect spatial substrate heterogeneities inside industrial bioreactors (whole-cell biosensor). Indeed, such heterogeneities cause a lowering of the biomass yield and an increase ofby-products concentration [1]. Three green fluorescent protein (GFP) transcriptional reporters have been chosen in E.coli, i.e. uspAr.gfp, csiE::gfp and yciGr.gfp. The promoter uspA is induced in response to a variety of stresses whereas the two other promoters, csiE and yciG, are supposed to be more specific in front of a substrate limitation. The responsiveness of these biosensors has been assessed in chemostat reactor. Secondly, the same biosensors have been tested in well-mixed laboratory reactors and in scale-down reactors able to reproduce industrial conditions. Finally, a mini scale-down platform has been proposed as a high throughput tool to investigate rapidly the usefulness of a given microbial biosensor. At the same time, GFP leakage from biosensors to extracellular medium was followed thanks to western blot analysis of supernatants and was correlated with membrane permeability.
机译：为了检测工业生物反应器（全细胞生物传感器）内部的空间基质异质性，已使用了对基质限制敏感的生物传感器。确实，这种异质性导致生物量产量的下降和副产物浓度的增加[1]。已在大肠杆菌中选择了三种绿色荧光蛋白（GFP）转录报告子，即uspAr.gfp，csiE :: gfp和yciGr.gfp。 uspA启动子是响应多种压力而诱导的，而另外两个启动子csiE和yciG则在底物限制之前更具特异性。这些生物传感器的响应能力已在恒化器反应器中进行了评估。其次，相同的生物传感器已经在充分混合的实验室反应器和能够再现工业条件的按比例缩小的反应器中进行了测试。最后，已经提出了一种微型缩减平台作为高通量工具来快速研究给定微生物生物传感器的实用性。同时，归因于上清液的蛋白质印迹分析，追踪了从生物传感器到细胞外培养基的GFP泄漏，并与膜通透性相关。

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