In order to assess the impact of crop rotation and nitrogen fertilisation in an agro system, a long-term field experiment has been established in 2006 at the experimental farm of Ghent University and University College Ghent (Bottelare - Belgium). Thetrial comprises 11 different crop rotations in combination with four nitrogen fertilizer regimes. The different crop rotations are monoculture of grain- and silage maize, whether or not followed by Italian ryegrass, permanent and temporary grass-cloverand six other rotations of maize in combination with potatoes, wheat, fodder beet and peas. Normal crop husbandry measures were taken for each crop.The experiment was set up on a sandy loam soil, according to a strip plot design with 3 replicates. In the course of the experiment, crop rotation was the horizontal factor and fertilizer nitrogen (N) the vertical factor. The effect of crop rotation on yield, disease pressure, soil structure and earthworm abundance were evaluated yearly.In autumn 2013 the weed seed bank was analysed for each plot using the seedling emergence method. The obtained results indicated differences between the different crop rotations.
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