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首页> 外文期刊>Communications in Agricultural and Applied Biological Sciences >THE POTENTIAL FOR USING ENTOMOPATHOGENIC NEMATODES TO CONTROL DARKWINGED FUNGUS GNATS BRADYSIA COPROPHILA (LINTNER) ON SUCCULENTS IN GLASS HOUSES
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THE POTENTIAL FOR USING ENTOMOPATHOGENIC NEMATODES TO CONTROL DARKWINGED FUNGUS GNATS BRADYSIA COPROPHILA (LINTNER) ON SUCCULENTS IN GLASS HOUSES

机译：利用昆虫内生线虫控制玻璃房中的微生物对潜伏的真菌性食肉芽孢杆菌慢生潜伏性（线虫）的潜力

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摘要

Dark winged fungus gnat Bradysia coprophila Lintner (Diptera: Sciaridae) is known as a pest of ornamental plants in commercial green and glass houses in nurseries worldwide. There have been reports about significant Bradysia coprophila damage of succulent plants in five Botanical gardens in Ukraine, including Academician Fomin's Botanical garden (Kiev) for the last three years. In pot experiments the commercial strain of 5. feltiae to control 8. coprophila was tested on Mammilaria, Opuntia, Echinocerus plants grown individually in plastic pots at rate 500 000 Us/ m2. Efficacy of EPN was evaluated based on the percentage of fly emergence from compost and captured in yellow sticky traps in treated and untreated benches with pots. It was shown that Steinernema feltiae application causes significant reduction (90%) of flies captured in yellow traps in comparison with the control. To evaluate virulence of EPNs to B. coprophila in laboratory bioassays, fourth instar larvae were exposed to 20,50,75,100 Us. B. coprophila was susceptible to all commercial and wild isolates of Steinernema and Heterorhabditis spp. Percentage mortality of B. coprophila larva ranged between 49 and 95%. Wild and commercial isolates of S. feltiae were highly virulent to the pest. The highest mortality - 95% was obtained by using a wild strain of S. feltiae. The nematode concentration tills to 50 IJ and all nematode species significantly affected the mortality rate of B. coprophila. Increasing the dosage of Steinernema spp. from75 to 100 Us did not affect significantly the mortality rate of the insect.
机译：深色翅真菌Bra（Bradysia coprophila Lintner）（双翅目：S虫科）是全世界苗圃中商业绿色和玻璃房屋中的观赏植物害虫。在过去的三年中，有报道称乌克兰的五个植物园中包括肉食植物的肉质植物对布雷迪西亚芽胞杆菌的严重破坏，包括弗明院士的植物园（基辅）。在盆栽实验中，在塑料盆中分别生长的乳木虱，仙人掌，棘皮E植物上以500000 Us / m2的速率测试了5.对照的8.亲液的商业菌株。 EPN的功效是根据堆肥中飞出的蝇的百分比进行评估的，并将其捕获在经过处理和未经处理的带盆工作台的黄色粘性陷阱中。结果表明，与对照相比，应用Steinernema feeliae可以显着减少捕获在黄色诱集装置中的苍蝇（90％）。为了在实验室生物测定中评估EPNs对B.coprophila的毒力，将四龄幼虫暴露于20,50,75,100 Us。丙酸杆菌对所有商业和野生分离的Steinernema和Heterorhabditis spp都很敏感。丙酸杆菌的幼虫死亡率在49％至95％之间。野生和商业分离株的S. feeliae对害虫具有高度毒性。通过使用野生的S. feeliae菌株获得最高的死亡率-95％。线虫的浓度直至50 IJ，所有线虫种类均显着影响了proprophila的死亡率。增加Steinernema spp的剂量。从75到100 Us并没有显着影响昆虫的死亡率。

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