• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Communications in Agricultural and Applied Biological Sciences >CHARACTERIZATION AND BIOCONTROL POTENTOF STREPTOMYCESSP. ISOLATED FROM THE RHIZOSPHERE OF ONONISANGUSTISSIMA LAM.
【24h】

CHARACTERIZATION AND BIOCONTROL POTENTOF STREPTOMYCESSP. ISOLATED FROM THE RHIZOSPHERE OF ONONISANGUSTISSIMA LAM.

机译：链霉菌的鉴定和生物防治潜力。分离自ONONISANGUSTISSIMA LAM的根际。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A total of 40 actinomycetes isolated from rhizosphere soils of Ononis angustissima Lam. were In vitro tested for their antagonism against deferent pathogenic microorganisms by streak assay. Among the isolates, four (21, 2A26, 1B10 and 2C34) present a potent antagonism against both pathogenic bacteria and fungi, they were selected, identified by 16S rDNA sequence analysis and phenotypic properties, and tested for their antimicrobial activity as well as their biocontrol potential against Chickpea (Cicer arietinum L.) pathogenic fungus [Fusarium oxysporum). Cultural characteristic studies strongly suggested that these strains belong to the genus Streptomyces. The four Streptomyces sp., solubilize phosphate and produce extracellular fungal cell-wall degrading enzymes chitinase and protease, as well as a marked production of acid p-indole acetic (AIA). The nucleotide sequence of thel6S rRNA gene of Streptomyces sp. strains 21,2A26,1B10 and 2C34 exhibited close similarity (62-75%) with Streptomyces parvulusMARS 16S rRNA genes. The inhibition was higher against fungi and Gram* bacteria, while Gram bacteria were less inhibited. The growth of the plant pathogenic fungus Fusarium oxysporum was considerably inhibited in the presence of the strains 21, 2A26, 1B10 and 2C34 culture supernatant. These studies revealed that the presence of the Streptomyces strains in the soil significantly promoted the growth of the Chickpea plants. These results indicate that the Streptomyces strains isolated for rhizosphere fromOnonis angustissima Lam. growing in arid conditions in southern Algeria (Sahara) could be an interesting source for antimicrobial bioactive substances and as biocontrol agents.
机译：从Ononis angustissima Lam的根际土壤中分离出总共40种放线菌。通过条纹试验在体外测试了它们对不同病原微生物的拮抗作用。在分离物中，有四个（21、2A26、1B10和2C34）对病原菌和真菌均具有较强的拮抗作用，对它们进行了选择，通过16S rDNA序列分析和表型特性进行了鉴定，并测试了它们的抗菌活性和生物防治作用。对鹰嘴豆（Cicer arietinum L.）致病性真菌[Fsarium oxysporum）具有潜在的抗药性。文化特性研究强烈提示这些菌株属于链霉菌属。四个链霉菌可溶解磷酸盐并产生细胞外真菌细胞壁降解酶几丁质酶和蛋白酶，并显着产生酸性对吲哚乙酸（AIA）。链霉菌sp16S rRNA基因的核苷酸序列。菌株21,2A26、1B10和2C34与小链霉菌MARS 16S rRNA基因表现出紧密相似性（62-75％）。对真菌和革兰氏*细菌的抑制作用较高，而对革兰氏细菌的抑制作用较小。在菌株21、2A26、1B10和2C34培养上清液的存在下，植物病原真菌尖孢镰刀菌的生长受到了显着抑制。这些研究表明，土壤中链霉菌菌株的存在显着促进了鹰嘴豆植物的生长。这些结果表明，从Ononis angustissima Lam的根际分离了链霉菌菌株。在阿尔及利亚南部（撒哈拉）干旱条件下生长的细菌可能成为抗菌生物活性物质和生物防治剂的有趣来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号