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首页> 外文期刊>Journal of Virological Methods >Detection of specific reticuloendotheliosis virus sequence and protein from REV-integrated fowlpox virus strains
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Detection of specific reticuloendotheliosis virus sequence and protein from REV-integrated fowlpox virus strains

机译：从整合REV的鸡痘病毒株中检测特定的网状内皮病病毒序列和蛋白质

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摘要

The detection is described of reticuloendotheliosis virus (REV) protein in tissue culture of chicken embryonated cells (CEFs) infected with field isolates of fowl poxvirus (FPV). By the polymerase chain reaction (PCR), five out of the six field isolates, but two out of the seven vaccine strains of FPV, were found to have had a 291 bp repeat sequence of REV-LTR integrated in their genomic DNA. An immunofluorescence (IF) method was employed using a monoclonal antibody (MAb) known to specify strain common envelope proteins for REV and allowed to detect the presence of a specific REV protein. The IF results indicate the localization of REV proteins in boundaries defined precisely within cells infected with these field strains of FPV carrying REV (FPV-REV). Furthermore, by immunoblotting (IB) using a chemiluminescent detection kit, the REV protein reacted specifically with the MAb and had a relative molecular mass (RMM) of 62 kDa. The data have the potential to advance substantially the current understanding of the integrated REV in FPV strains; and the identification of a unique protein associated with variant forms of FPV will also offer great potential for identification of novel vaccine candidates for use in poultry against variant forms of FPV.
机译：描述了网状内皮内皮病病毒（REV）蛋白在鸡痘病毒（FPV）田间分离株感染的鸡胚细胞（CEF）组织培养物中的检测。通过聚合酶链反应（PCR），发现六种田间分离株中有五种，但七种FPV疫苗株中有两株在其基因组DNA中整合了一个291 bp的REV-LTR重复序列。使用单克隆抗体（MAb）的免疫荧光（IF）方法，已知该单克隆抗体可指定REV的菌株常见包膜蛋白并允许检测特定REV蛋白的存在。 IF结果表明，REV蛋白的定位精确限定在感染了带有REV（FPV-REV）的FPV田间菌株的细胞内。此外，通过使用化学发光检测试剂盒的免疫印迹（IB），REV蛋白与MAb特异性反应，相对分子质量（RMM）为62 kDa。这些数据有可能大大促进当前对FPV菌株中整合REV的了解。与FPV变体形式相关的独特蛋白质的鉴定也将为鉴定用于FPV变体形式的家禽中的新型候选疫苗提供巨大潜力。

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