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首页> 外文期刊>Journal of Virological Methods >International interlaboratory trials on rabies diagnosis: an overview of results and variation in reference diagnosis techniques (fluorescent antibody test, rabies tissue culture infection test, mouse inoculation test) and molecular biology techniques.
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International interlaboratory trials on rabies diagnosis: an overview of results and variation in reference diagnosis techniques (fluorescent antibody test, rabies tissue culture infection test, mouse inoculation test) and molecular biology techniques.

机译：狂犬病诊断的国际实验室间试验：参考诊断技术（荧光抗体试验，狂犬病组织培养物感染试验，小鼠接种试验）和分子生物学技术的结果和变化概述。

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Interlaboratory trials on rabies diagnosis were organised in 2009 and in 2010 by the European Union Reference Laboratory (EURL) for rabies. In 2009, two panels of virus samples were sent to participating laboratories to compare results on reference diagnosis techniques and on RT-PCR. A single panel was sent in 2010 to test FAT (fluorescent antibody test), RTCIT (rabies tissue culture infection test) and RT-PCR techniques. The virus panels included the RABV, EBLV-1, EBLV-2 and ABLV strains. Results revealed that laboratories produced the highest proportion of concordant results using RT-PCR (90.5%) and FAT (87.1%), followed by RTCIT (70.0%) and MIT (35.0%) in 2009 and in FAT (85.0%) and RT-PCR (80.6%) followed by RTCIT (77.3%) in 2010. Errors were only observed in bat strains (i.e. none in the RABV strain) for the RT-PCR or FAT techniques, highlighting the need to improve diagnosis most specifically in such strains. RT-PCR was the technique showing the lowest rate of false negative results in either trial year, while RTCIT and MIT (performed in 2009 only) were the techniques with the lowest proportion of false positive results. Nevertheless, the FAT technique represented a good compromise with both satisfactory sensitivity and specificity, as only a few false positive (1.6% in 2009, 5.8% in 2010) and false negative results (1.6% in both 2009 and 2010) were detected. The analysis of technical questionnaires describing the protocols used by participating laboratories revealed variation in the methods used that may induce inconsistencies in the results. In this study, the number of readers for FAT slide examination was identified as a factor affecting significantly the results of laboratories, suggesting that two independent readers are necessary for routine rabies diagnosis. Our findings highlight the need for all rabies diagnostic laboratories to improve harmonisation of procedures.Digital Object Identifier http://dx.doi.org/10.1016/j.jviromet.2011.06.004
机译：欧盟狂犬病参考实验室（EURL）于2009年和2010年组织了狂犬病诊断实验室间试验。 2009年，将两批病毒样本发送到参与实验室，以比较参考诊断技术和RT-PCR的结果。 2010年发送了一个小组来测试FAT（荧光抗体测试），RTCIT（狂犬病组织培养感染测试）和RT-PCR技术。病毒组包括RABV，EBLV-1，EBLV-2和ABLV株。结果显示，实验室使用RT-PCR（90.5％）和FAT（87.1％）产生的一致性最高，2009年依次是RTCIT（70.0％）和MIT（35.0％），以及FAT（85.0％）和RT -PCR（80.6％），其次是RTCIT（77.3％），在2010年。对于RT-PCR或FAT技术，仅在蝙蝠菌株中观察到错误（即RABV菌株中没有），突出显示了在这种情况下最需要改进诊断的需求株。 RT-PCR是在任何一个试验年度中假阴性结果发生率最低的技术，而RTCIT和MIT（仅在2009年执行）是假阳性结果发生率最低的技术。尽管如此，FAT技术还是在令人满意的敏感性和特异性上取得了很好的折衷，因为仅检测到了少数假阳性（2009年为1.6％，2010年为5.8％）和假阴性结果（2009年和2010年均为1.6％）。对参与实验室使用的协议进行技术问卷调查的分析表明，所用方法的差异可能导致结果不一致。在这项研究中，FAT幻灯片检查的读者人数被确定为显着影响实验室结果的因素，这表明常规狂犬病诊断需要两个独立的读者。我们的发现强调了所有狂犬病诊断实验室都需要改善程序的统一性。数字对象标识符http://dx.doi.org/10.1016/j.jviromet.2011.06.004

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