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首页> 外文期刊>Journal of Virological Methods >Degenerate primers based RT-PCR for rapid detection and differentiation of airborne chicken Newcastle disease virus in chicken houses.
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Degenerate primers based RT-PCR for rapid detection and differentiation of airborne chicken Newcastle disease virus in chicken houses.

机译：基于简并引物的RT-PCR快速检测和区分鸡舍中的机载鸡新城疫病毒。

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摘要

Airborne Newcastle disease (ND) viruses in the air of five chicken houses were detected and differentiated by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers. Fifteen air samples were collected with All Glass Impinger-30 (AGI-30) air samplers in each house. Airborne ND viruses were also isolated and virulence identified by in vivo tests. Avirulent viruses were detected both in air samples and swab samples in four houses by degenerate primers based RT-PCR. Virulent viruses were detected only in the air samples by degenerate primers based RT-PCR in two houses. Seven strains viruses were isolated from the RT-PCR positive air samples. Of the seven strains, three strains were virulent viruses and four strains were avirulent viruses identified by in vivo tests. The results showed that it was feasible to detect and differentiate NDV in the air samples using degenerate primers based RT-PCR. This technique could decrease the time it required identify NDV infected flocks while distinguishing between virulent and avirulent viruses. It will help effectively to control Newcastle disease.
机译：使用简并引物通过逆转录酶聚合酶链反应（RT-PCR）检测并区分了五个鸡舍中的空气传播的新城疫（ND）病毒。在每个房屋中使用All Glass Impinger-30（AGI-30）空气采样器收集了15个空气样本。还分离了机载ND病毒，并通过体内测试鉴定了毒力。通过基于简并引物的RT-PCR，在四个房屋的空气样本和拭子样本中均检测到无毒病毒。通过简并引物基于RT-PCR在两个房屋中仅在空气样本中检测到了强毒病毒。从RT-PCR阳性空气样品中分离出7种病毒。在七个菌株中，三个菌株是强毒病毒，四个菌株是通过体内试验鉴定的无毒病毒。结果表明，使用基于简并引物的RT-PCR检测和区分空气样品中的NDV是可行的。这项技术可以减少识别NDV感染鸡群所需的时间，同时区分有毒和无毒病毒。这将有助于有效控制新城疫。

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