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首页> 外文期刊>Journal of Zoo and Wildlife Medicine >Evidence of Leishmania spp. antibodies and DNA in bush dogs (Speothos venaticus) in Brazil
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Evidence of Leishmania spp. antibodies and DNA in bush dogs (Speothos venaticus) in Brazil

机译:利什曼原虫属的证据。巴西丛林犬(Speothos venaticus)的抗体和DNA

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摘要

The municipality of Ilha Solteira, Sao Paulo, Brazil is an endemic area of leishmaniasis. At the Companhia Energetica de Sao Paulo (CESP) Wild Animal Center of Ilha Solteira, two bush dogs (Speothos venaticus) showed clinical signs of this disease. The amastigote form of Leishmania was detected in lymph-node smears taken by fine-needle biopsy. In addition, serum samples from both animals, screened with an enzyme-linked immunosorbent assay (ELISA), were positive for anti-Leishmania antibodies. Moreover, tissue samples from one of the bush dogs were evaluated for the presence of Leishmania DNA by means of a polymerase chain reaction (PCR). DNA of the parasite was indeed detected in the tissue samples of the liver and the lymph nodes; however, no DNA from the parasite was detected in samples of the skin and spleen. These findings confirm a Leishmania infection in bush dogs (S. venaticus).
机译:巴西圣保罗的Ilha Solteira市是利什曼病的流行地区。在Ilha Solteira的Companhia Energetica de Sao Paulo(CESP)野生动物中心,两只野狗(Speothos venaticus)表现出这种疾病的临床症状。在细针穿刺活检的淋巴结涂片中检测到利什曼原虫的鞭毛体形式。此外,用酶联免疫吸附测定(ELISA)筛选的两只动物的血清样品均显示抗利什曼原虫抗体呈阳性。此外,通过聚合酶链反应(PCR)评估了来自其中一只灌木狗的组织样品中利什曼原虫DNA的存在。确实在肝脏和淋巴结的组织样本中检测到了该寄生虫的DNA。但是,在皮肤和脾脏样本中未检测到来自寄生虫的DNA。这些发现证实了布什犬(S. venaticus)中的利什曼原虫感染。

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