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首页> 外文期刊>Journal of Zoological Systematics and Evolutionary Research >Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics
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Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics

机译：使用shot弹枪蛋白质组学研究处于活跃状态与无水状态的缓速菌的热激蛋白

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摘要

The eutardigrade Milnesium tardigradum can undergo cryptobiosis and can survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood. Heat shock proteins (Hsps) as an important subgroup of chaperones which protect proteins from irreversible aggregation and degradation might play an important role in anhydrobiosis. In this report, we therefore investigated Hsps in tardigrades in the active versus the anhydrobiotic state employing proteomics techniques. Protein lysates from tardigrades in both states were separated by one-dimensional gel electrophoresis, in-gel digested with trypsin and tryptic peptides were analyzed by high sensitive nanoLC-ESI-MS/MS on a LTQ-Orbitrap mass spectrometer. This study indicates the presence of Hsps of the major chaperone families Hsp40, Hsp60, Hsp70, Hsp90, small Hsps and furthermore nucleotide exchange factors and co-chaperones in Milnesium tardigradum. A comparative analysis of the identified Hsps in both states was performed by calculating the exponentially modified protein abundance index.
机译：准原种的Miltarium tardigradum可以发生隐生菌，并且可以在极端的环境条件下生存。然而，关于缓步动物的生存机制仍然知之甚少。热激蛋白（Hsps）作为保护蛋白免于不可逆聚集和降解的伴侣的重要子类，可能在脱水生物中起重要作用。因此，在本报告中，我们使用蛋白质组学技术研究了活性状态与无水状态下缓坡中的Hsps。通过一维凝胶电泳分离来自两种状态的节肢动物的蛋白裂解物，用胰蛋白酶在凝胶中消化，并通过高灵敏度nanoLC-ESI-MS / MS在LTQ-Orbitrap质谱仪上分析胰蛋白酶肽。这项研究表明在米尔德米尔草中主要伴侣分子Hsp40，Hsp60，Hsp70，Hsp90，小Hsps以及其他核苷酸交换因子和共伴侣的Hsps的存在。通过计算指数修饰的蛋白质丰度指数，对两种状态下鉴定出的Hsps进行了比较分析。

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