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首页> 外文期刊>Journal of the Association for Research in Otolaryngology: JARO >Systemic lipopolysaccharide compromises the blood-labyrinth barrier and increases entry of serum fluorescein into the perilymph
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Systemic lipopolysaccharide compromises the blood-labyrinth barrier and increases entry of serum fluorescein into the perilymph

机译：全身性脂多糖破坏了血液的迷宫屏障并增加了血清荧光素进入外周淋巴的进入

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摘要

The blood vessels that supply the inner ear form a barrier between the blood and the inner ear fluids to control the exchange of solutes, protein, and water. This barrier, called the blood-labyrinth barrier (BLB) is analogous to the blood-brain barrier (BBB), which plays a critical role in limiting the entry of inflammatory and infectious agents into the central nervous system. We have developed an in vivo method to assess the functional integrity of the BLB by injecting sodium fluorescein into the systemic circulation of mice and measuring the amount of fluorescein that enters perilymph in live animals. In these experiments, perilymph was collected from control and experimental mice in sequential samples taken from the posterior semicircular canal approximately 30 min after systemic fluorescein administration. Perilymph fluorescein concentrations in control mice were compared with perilymph fluorescein concentrations after lipopolysaccharide (LPS) treatment (1 mg/kg IP daily for 2 days). The concentration of perilymphatic fluorescein, normalized to serum fluorescein, was significantly higher in LPS-treated mice compared to controls. In order to assess the contributions of perilymph and endolymph in our inner ear fluid samples, sodium ion concentration of the inner ear fluid was measured using ion-selective electrodes. The sampled fluid from the posterior semicircular canal demonstrated an average sodium concentration of 145 mM, consistent with perilymph. These experiments establish a novel technique to assess the functional integrity of the BLB using quantitative methods and to provide a comparison of the BLB to the BBB.
机译：供应内耳的血管在血液和内耳液之间形成屏障，以控制溶质，蛋白质和水的交换。这种称为血迷宫屏障（BLB）的屏障类似于血脑屏障（BBB），它在限制炎症和感染性物质进入中枢神经系统中起着关键作用。我们已经开发了一种体内方法，通过将荧光素钠注射到小鼠的体循环中并测量进入活体动物淋巴中的荧光素的量来评估BLB的功能完整性。在这些实验中，在全身性荧光素给药后约30分钟，从对照组和实验小鼠的后半圆形管中采集的顺序样本中收集了淋巴。将对照小鼠中的外周淋巴荧光素浓度与脂多糖（LPS）处理（每天1 mg / kg IP，持续2天）后的外周淋巴荧光素浓度进行比较。与对照组相比，LPS处理的小鼠中，以血清荧光素标准化的淋巴周荧光素浓度明显更高。为了评估在我们的内耳液样本中淋巴和内淋巴液的贡献，使用离子选择电极测量了内耳液的钠离子浓度。从后半圆形管中取样的液体显示平均钠浓度为145 mM，与淋巴液一致。这些实验建立了一种新颖的技术，可使用定量方法评估BLB的功能完整性，并提供BLB与BBB的比较。

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