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首页> 外文期刊>Diabetes/metabolism research and reviews >β-Carotene, α-tocopherol and ascorbic acid: Differential profile of antioxidant, inflammatory status and regulation of gene expression in human mononuclear cells of diabetic donors
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β-Carotene, α-tocopherol and ascorbic acid: Differential profile of antioxidant, inflammatory status and regulation of gene expression in human mononuclear cells of diabetic donors

机译：β-胡萝卜素，α-生育酚和抗坏血酸：糖尿病供体人单核细胞中抗氧化剂，炎症状态和基因表达调控的差异性

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Background: Diabetic patients are exposed to increased oxidative stress due to several mechanisms, mainly hyperglycaemia. Pathological processes, such as those in type 1 diabetes, include diminished activity of the antioxidant defense system(s) or excessive oxidative generation resulting in an oxidative/antioxidant imbalance and development of oxidative stress. Methods: The purpose of this study was to evaluate the production of reactive oxygen species (ROS) (chemiluminescence) and reduction capacity (MTT dye reduction), the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits, superoxide dismutase and catalase using quantitative reverse-transcriptase polymerase chain reaction, and the levels of cytokines [interleukin (IL)-6, tumour necrosis factor-α, IL-8, IL-10 and IL-4] by sandwich enzyme-linked immunosorbent assay in mononuclear cells from non-diabetic and diabetic donors treated with a vitamin complex (ascorbic acid, β-carotene and α-tocopherol) in two different concentrations ([A]=ascorbic acid=0.08μM, α-tocopherol=0.04μM, β-carotene=0.0008μM and [20A]=ascorbic acid=1.6μM, α-tocopherol=0.82μM, β-carotene=0.016μM). Results: Concentration [A] was antioxidant reducing ROS production, expression of NADPH oxidase subunits and pro-inflammatory cytokines while raising the expression of antioxidant enzymes and reducing pro-inflammatory cytokines in both groups. Concentration [20A] was pro-oxidant by raising ROS production, NADPH oxidase subunits and pro-inflammatory cytokines and reducing antioxidant enzymes and anti-inflammatory cytokines in the non-diabetic group but antioxidant in cells of type 1 diabetic patients by raising antioxidant enzymes and anti-inflammatory cytokines and reducing pro-inflammatory cytokines. Conclusion: The vitamin complex has a dual effect, pro-oxidant and antioxidant, being also dose dependent with different profiles of cells of non-diabetic and type 1 diabetic patients.
机译：背景：糖尿病患者由于多种机制（主要是高血糖症）而暴露于氧化应激增加。诸如1型糖尿病的病理过程包括抗氧化剂防御系统的活性降低或过度的氧化生成，从而导致氧化/抗氧化剂失衡和氧化应激的发展。方法：本研究的目的是使用定量方法评估活性氧（ROS）的产生（化学发光）和还原能力（MTT染料还原），烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶亚单位，超氧化物歧化酶和过氧化氢酶的表达。夹心酶联免疫吸附法在非单核细胞中进行逆转录酶聚合酶链反应和细胞因子[白介素（IL）-6，肿瘤坏死因子-α，IL-8，IL-10和IL-4]的水平维生素复合物（抗坏血酸，β-胡萝卜素和α-生育酚）以两种不同浓度（[A] =抗坏血酸=0.08μM，α-生育酚=0.04μM，β-胡萝卜素=0.0008μM）处理的糖尿病和糖尿病供体[20A] ＝抗坏血酸＝1.6μM，α-生育酚＝0.82μM，β-胡萝卜素＝0.016μM）。结果：浓度[A]是抗氧化剂，可降低ROS的产生，NADPH氧化酶亚基和促炎细胞因子的表达，同时提高两组抗氧化酶的表达并减少促炎细胞因子。浓度[20A]通过增加ROS的产生，NADPH氧化酶亚基和促炎性细胞因子并减少非糖尿病组中的抗氧化剂和抗炎性细胞因子而成为促氧化剂，但通过提高抗氧化剂和1型糖尿病患者细胞中的抗氧化剂来提高其浓度。抗炎细胞因子和减少促炎细胞因子。结论：维生素复合物具有双重作用，即抗氧化剂和抗氧化剂，并且与非糖尿病和1型糖尿病患者的细胞特征不同，剂量依赖性。

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