...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of interferon and cytokine research: The official journal of the International Society for Interferon and Cytokine Research >IFN-alpha1 plasmid construct affords protection against HSV-1 infection in transfected L929 fibroblasts.
【24h】

IFN-alpha1 plasmid construct affords protection against HSV-1 infection in transfected L929 fibroblasts.

机译：IFN-alpha1质粒构建体可抵抗转染的L929成纤维细胞中的HSV-1感染。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of the present study was to evaluate the resistance against herpes simplex virus type 1 (HSV-1) using an interferon-alpha1 (IFN-alpha1) transgene in specifically targeted cells in vitro. Transfection of mouse fibroblast L929 cells with an IFN-alpha1 plasmid construct reduced viral load and viral gene expression in a time-dependent fashion. Supernatants from IFN-alpha1-transfected cells augmented natural killer (NK) cell activity, and such an effect was antagonized with neutralizing antibody to IFN-alpha/beta. In addition, transfected cells displayed an increase in the IFN inducible genes (2',5'-oligoadenylate synthetase [2',5'-OAS], T cell-specific guanine nucleotide triphosphate-binding protein, IFN regulatory factor 1 [IRF-1], and major histocompatibility complex [MHC] class I) compared with plasmid vector-treated controls. Collectively, these results show that IFN-alpha1 transfection of cells in vitro induces or upregulates a spectrum of IFN-regulated genes involved in the direct or indirect antiviral action of this cytokine. In addition, the transgene significantly increases the resistance of transfected cells in vitro to HSV-1 infection.
机译：本研究的目的是在体外特异性靶向细胞中使用干扰素-α1（IFN-α1）转基因评估对1型单纯疱疹病毒（HSV-1）的耐药性。用IFN-alpha1质粒构建体转染小鼠成纤维细胞L929细胞可减少病毒载量和病毒基因表达，并具有时间依赖性。来自IFN-alpha1转染细胞的上清液增强了自然杀伤（NK）细胞的活性，而这种作用被针对IFN-alpha / beta的中和抗体所拮抗。此外，转染的细胞显示出IFN诱导型基因（2'，5'-寡腺苷酸合成酶[2'，5'-OAS]，T细胞特异性鸟嘌呤核苷酸三磷酸结合蛋白，IFN调节因子1 [IRF- 1]，以及与质粒载体处理的对照相比的主要组织相容性复合物[MHC] I类）。总而言之，这些结果表明，体外干扰素-α1细胞转染诱导或上调参与该细胞因子直接或间接抗病毒作用的一系列干扰素调节基因。另外，转基因显着增加了体外转染细胞对HSV-1感染的抗性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号