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首页> 外文期刊>Journal of cellular biochemistry. >Androgen and taxol cause cell type-specific alterations of centrosome and DNA organization in androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cells.
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Androgen and taxol cause cell type-specific alterations of centrosome and DNA organization in androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cells.

机译：雄激素和紫杉醇引起雄激素反应性LNCaP和非雄激素依赖性DU145前列腺癌细胞中中心体和DNA组织的细胞类型特异性改变。

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We investigated the effects of androgen and taxol on the androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cell lines. Cells were treated for 48 and 72 h with 0.05-1 nM of the synthetic androgen R1881 and with 100 nM taxol. Treatment of LNCaP cells with 0.05 nM R1881 led to increased cell proliferation, whereas treatment with 1 nM R1881 resulted in inhibited cell division, DNA cycle arrest, and altered centrosome organization. After treatment with 1 nM R1881, chromatin became clustered, nuclear envelopes convoluted, and mitochondria accumulated around the nucleus. Immunofluorescence microscopy with antibodies to centrosomes showed altered centrosome structure. Although centrosomes were closely associated with the nucleus in untreated cells, they dispersed into the cytoplasm after treatment with 1 nM R1881. Microtubules were only faintly detected in 1 nM R1881-treated LNCaP cells. The effects of taxol included microtubule bundling and altered mitochondria morphology, but not DNA organization. As expected, the androgen-independent prostate cancer cell line DU145 was not affected by R1881. Treatment with taxol resulted in bundling of microtubules in both cell lines. Additional taxol effects were seen in DU145 cells with micronucleation of DNA, an indication of apoptosis. Simultaneous treatment with R1881 and taxol had no additional effects on LNCaP or DU145 cells. These results suggest that LNCaP and DU145 prostate cancer cells show differences not only in androgen responsiveness but in sensitivity to taxol as well. Copyright 2000 Wiley-Liss, Inc.
机译：我们调查了雄激素和紫杉醇对雄激素反应性LNCaP和非雄激素依赖性DU145前列腺癌细胞系的影响。用0.05-1 nM的合成雄激素R1881和100 nM紫杉醇处理细胞48和72小时。用0.05 nM R1881处理LNCaP细胞会导致细胞增殖增加，而使用1 nM R1881处理会抑制细胞分裂，DNA周期停滞并改变中心体组织。用1 nM R1881处理后，染色质聚集，核被膜回旋，线粒体聚集在核周围。用抗中心体抗体的免疫荧光显微镜检查显示了中心体结构的改变。尽管中心体在未经处理的细胞中与细胞核密切相关，但在用1 nM R1881处理后它们分散到细胞质中。仅在1 nM R1881处理的LNCaP细胞中微弱地检测到微管。紫杉醇的作用包括微管捆绑和改变的线粒体形态，但不包括DNA组织。如预期的那样，非雄激素依赖性前列腺癌细胞系DU145不受R1881的影响。用紫杉醇处理导致两种细胞系中微管的束缚。在具有DNA微核的DU145细胞中观察到其他紫杉醇效应，这是细胞凋亡的迹象。用R1881和紫杉醇同时治疗对LNCaP或DU145细胞没有其他影响。这些结果表明，LNCaP和DU145前列腺癌细胞不仅在雄激素反应性上而且在对紫杉醇的敏感性上也表现出差异。版权所有2000 Wiley-Liss，Inc.

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