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首页> 外文期刊>Journal of cellular biochemistry. >N-acetylcysteine increases susceptibility of HeLa cells to bacterial invasion
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N-acetylcysteine increases susceptibility of HeLa cells to bacterial invasion

机译：N-乙酰半胱氨酸增加HeLa细胞对细菌入侵的敏感性

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摘要

Serratia grimesii are non-pathogenic bacteria capable, however, to invade eukaryotic cells provided that they synthesize intracellular metalloprotease grimelysin (Bozhokina et al. [2011] Cell. Biol. Int. 35: 111-118). To elucidate how invasion of grimelysin containing bacteria depends on physiological state of host cells, we studied the effect of N-acetylcysteine (NAC) on susceptibility of HeLa cells to invasion by the wild-type S. grimesii and recombinant E. coli expressing grimelysin gene. Incubation of HeLa cells with 10 mM NAC resulted in changes of cell morphology and disassembly of actin cytoskeleton that were reversed when NAC was removed from the culture medium. Both in the presence of NAC and upon its removal, the entry of grimelysin producing bacteria increased by a factor of 1.5-2 and 3-3.5 for wild-type S. grimesii and recombinant E. coli, respectively. This effect does not correlate with cytoskeleton rearrangements but may be due to the NAC-induced up-regulation of cell surface receptors playing a role in cell adhesion and cell-cell junctions. A twofold difference in the efficiency of S. grimesii and recombinant E. coli to enter the NAC-treated cells suggests that the entry of the wild-type and recombinant bacteria occurs via different receptors which activity is differently affected by NAC.
机译：格氏沙雷氏菌是能够侵入真核细胞的非病原性细菌，只要它们能够合成细胞内金属蛋白酶格莱姆霉素（Bozhokina等人，[2011] Cell.Biol.Int.35：111-118）。为了阐明含格莱姆菌的细菌的入侵如何取决于宿主细胞的生理状态，我们研究了N-乙酰半胱氨酸（NAC）对野生型格氏杆菌和表达格莱姆菌素基因的重组大肠杆菌侵袭Hela细胞敏感性的影响。 。将HeLa细胞与10 mM NAC一起孵育会导致细胞形态变化和肌动蛋白细胞骨架解体，当从培养基中去除NAC时，这种变化会逆转。在NAC存在下和去除NAC后，对于野生型格氏链球菌和重组大肠杆菌，产生格莱姆霉素的细菌的进入分别增加了1.5-2和3-3.5倍。此作用与细胞骨架重排无关，但可能是由于NAC诱导的细胞表面受体上调在细胞粘附和细胞-细胞连接中起作用。格氏链球菌和重组大肠杆菌进入NAC处理细胞的效率有两倍差异，表明野生型和重组细菌的进入通过不同的受体发生，而这些受体的活性受到NAC的影响不同。

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