...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular biochemistry. >Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47.
【24h】

Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47.

机译：FHL2（一种在人心脏中优先表达的LIM域蛋白）与hCDC47的蛋白相互作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the yeast two-hybrid library screening, the heart-specific FHL2 protein was found to interact with hCDC47. In vitro interaction study between FHL2 protein and hCDC47 was demonstrated. From the results of domain studies by the yeast two-hybrid assay, the second and third LIM domains in conjunction with the first half LIM domain of FHL2 were identified to be important in binding with hCDC47. Besides, in Northern blot hybridization of human cancer cell lines, the highest FHL2 mRNA expression was detected in colorectal adenocarcinoma SW480 and HeLa cell S3. Our results imply that FHL2 protein may associate with cancer development and may act as a molecular adapter to form a multicomplex with hCDC47 in the nucleus, thus it plays an important role in the specification or maintenance of the terminal differentiated phenotype of heart muscle cells. Copyright 2000 Wiley-Liss, Inc.
机译：在酵母双杂交文库筛选中，发现心脏特异性FHL2蛋白与hCDC47相互作用。证明了FHL2蛋白与hCDC47之间的体外相互作用研究。根据酵母双杂交测定的域研究结果，第二和第三LIM域以及FHL2的前半LIM域被确定对与hCDC47结合很重要。此外，在人癌细胞系的Northern印迹杂交中，在结直肠腺癌SW480和HeLa细胞S3中检测到最高的FHL2mRNA表达。我们的结果表明，FHL2蛋白可能与癌症的发展有关，并可能充当分子适配器，与hCDC47在细胞核中形成复合物，因此在规范或维持心肌细胞终末分化表型方面起着重要作用。版权所有2000 Wiley-Liss，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号