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首页> 外文期刊>Journal of cellular biochemistry. >Proline-rich transcript of the brain (prtb) is a serum-responsive gene in osteoblasts and upregulated during adhesion.
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Proline-rich transcript of the brain (prtb) is a serum-responsive gene in osteoblasts and upregulated during adhesion.

机译：富含脯氨酸的大脑转录物（prtb）是成骨细胞中的血清反应基因，在粘附过程中被上调。

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To characterize the temporal expression of genes that play a functional role during the process of osteoblast adhesion, we used differential display (DD-PCR) on mRNA isolated from attached vs. suspended osteoblasts. A 200-bp fragment displaying upregulated expression after 30 and 60 min adhesion was isolated, sequenced, and showed 97% homology to prtb, previously showed to be expressed in mouse brain. Northern analysis confirmed a two-fold increase in prtb message during adhesion to tissue culture polystyrene, both in the presence or absence of surface-adsorbed serum proteins. Serum stimulation alone was also able to induce prtb expression, although to a lesser extent, in suspension cells. Strong prtb expression was also detected in both brain and bone of adult rats. Furthermore, prtb expression analysis during MC3T3-E1 cell differentiation revealed high expression levels independent of proliferation (day 0-7), matrix maturation (day 7-14), and mineralization (day 14-31). Time course analysis of prtb expression during adhesion of sensitized osteoblasts to serum-protein coated surfaces showed robust mRNA expression at 5 min post-plating and a peak at 10 min. The two known serum-inducible immediate early genes c-fos and c-jun showed similar expression kinetics, with c-jun mRNA levels peaking at 15 min and c-fos at 20 min. Based on these data, we hypothesize that prtb may function as an immediate early, serum-responsive, and adhesion-inducible gene with possible involvement in processes such as cell cycle control, adhesion, and proliferation.
机译：为了表征在成骨细胞粘附过程中发挥功能作用的基因的时间表达，我们对从附着的与悬浮的成骨细胞分离的mRNA进行了差异显示（DD-PCR）。分离，测序并在粘附后30和60分钟后显示表达上调的200 bp片段进行测序，显示与prtb的同源性为97％，先前证明在小鼠脑中表达。 Northern分析证实在存在或不存在表面吸附的血清蛋白的情况下，在粘附于组织培养物聚苯乙烯过程中，prtb信息增加了两倍。单独的血清刺激也能够诱导prtb表达，尽管程度较小。在成年大鼠的大脑和骨骼中也检测到了强大的prtb表达。此外，在MC3T3-E1细胞分化过程中的prtb表达分析表明，高表达水平与增殖（第0-7天），基质成熟（第7-14天）和矿化（第14-31天）无关。在致敏的成骨细胞粘附于血清蛋白包被的表面期间，prtb表达的时程分析显示，在铺板后5分钟时鲁棒的mRNA表达，在10分钟时达到峰值。两个已知的血清诱导的立即早期基因c-fos和c-jun显示相似的表达动力学，其中c-jun mRNA水平在15分钟达到峰值，而c-fos在20分钟达到峰值。基于这些数据，我们假设prtb可能起着立即早期，血清反应性和粘附诱导性基因的作用，并可能参与细胞周期控制，粘附和增殖等过程。

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