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首页> 外文期刊>Journal of cellular biochemistry. >Lipogenic Enzymes Complexes and Cytoplasmic Lipid Droplet Formation During Adipogenesis
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Lipogenic Enzymes Complexes and Cytoplasmic Lipid Droplet Formation During Adipogenesis

机译：脂肪形成过程中的脂肪酶复合物和细胞质脂质液滴形成。

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Lipid droplets are dynamic organelles that store triglycerides and participate in their mobilization in adipose cells. These organelles require the reorganization of some structural components, the cytoskeleton, and the activation of lipogenic enzymes. Using confocal microscopy, we analyzed the participation of cytoskeletal components and two lipogenic enzymes, fatty acid synthase and glycerophosphate dehydrogenase, during lipid droplet biogenesis in differentiating 3T3-F442A cells into adipocytes. We show that subcortical actin microfilaments are extended at the basal side of the cells in parallel arrangement to the culture dish substrate, and that the microtubule network traverses the cytoplasm as a scaffold that supports the round shape of the mature adipocyte. By immunoprecipitation, we show that vimentin and perilipin1a associate during the early stages of the differentiation process for lipid droplet formation. We also report that the antibody against perilipin1 detected a band that might correspond to a modified form of the molecule. Finally, the cytosolic distribution and punctate organization of lipogenic enzymes and their co-localization in the proximity of lipid droplets suggest the existence of dynamic protein complexes involved in synthesis and storage of triglycerides. J. Cell. Biochem. 117: 2315-2326, 2016. (c) 2016 Wiley Periodicals, Inc.
机译：脂质小滴是动态的细胞器，可储存甘油三酸酯并参与其在脂肪细胞中的动员。这些细胞器需要一些结构成分的重组，细胞骨架和脂肪酶的激活。使用共聚焦显微镜，我们分析了在将3T3-F442A细胞分化为脂肪细胞的脂质小滴生化过程中细胞骨架成分和两种脂肪酶，脂肪酸合酶和甘油磷酸脱氢酶的参与。我们显示皮质下肌动蛋白微丝在细胞的基端以平行排列的方式延伸到培养皿底物，并且微管网络作为支持成熟脂肪细胞圆形的支架横越细胞质。通过免疫沉淀，我们显示波形蛋白和perilipin1a关联在脂质液滴形成分化过程的早期。我们还报告说，针对periplipin1的抗体检测到一条条带，该条带可能对应于该分子的修饰形式。最后，脂肪酶的胞质分布和点状组织及其在脂质小滴附近的共定位表明存在动态蛋白质复合物，该复合物涉及甘油三酸酯的合成和储存。 J.细胞。生化。 117：2315-2326，2016.（c）2016威利期刊公司

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