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首页> 外文期刊>Journal of cellular biochemistry. >Transcription factor pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: identification of two pit-1 genes in the carp.
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Transcription factor pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: identification of two pit-1 genes in the carp.

机译：转录因子pit-1的表达在季节性热适应过程中受到调节：对鲤鱼的两个pit-1基因进行鉴定。

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摘要

A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer-acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer-acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.
机译：鉴定并完整测序了鲤鱼的第二个Pit-1基因，包括完整的结构基因和1.1 kb的启动子区域。确定了外显子-内含子的结构，逆转录聚合酶链反应（RT-PCR）实验表明鲤鱼垂体中仅存在一种Pit-1剪接变体。在夏季和冬季适应的鲤鱼中研究了季节适应对Pit-1基因表达程度的影响。定量RT-PCR分析显示，与冬季适应的鱼类相比，夏季适应的鲤鱼垂体中Pit-1 mRNA明显增加。垂体腺切片与代表鲤鱼Pit-1完整5'-反式激活区的核糖核蛋白的原位杂交表明，在夏季适应的鱼的喙尖远端，Pit-1 mRNA水平明显升高，其中催乳素的表达方式为类似于远端近端和中间椎间观察到的季节性增加。 Pit-1的细胞和时间特异性转录支持其在分子机制中的作用，该分子机制是由于季节性变化对其栖息地的物理影响而导致的赤热鱼的适应过程的基础。

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