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首页> 外文期刊>Journal of cellular biochemistry. >Antimicrobials and in vitro systems: antibiotics and antimycotics alter the proteome of MCF-7 cells in culture.
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Antimicrobials and in vitro systems: antibiotics and antimycotics alter the proteome of MCF-7 cells in culture.

机译：抗生素和体外系统：抗生素和抗真菌药会改变培养物中MCF-7细胞的蛋白质组。

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Cell culture is widely used to study gene or protein changes in response to experimental conditions. The value of such experiments depends on stringent control and understanding of the in vitro environment. Despite well-documented evidence describing toxic effects in the clinical setting, antibiotics and antimycotics are routinely used in cell culture without regard for their potential toxicity. We cultured MCF-7 breast cancer cells in the presence/absence of antibiotics (penicillin/streptomycin) and/or the antimycotic amphotericin B. Differential protein expression was assessed using 2D-DIGE and MALDI-MS/MS. Antibiotics caused 8/488 spots (1.3% of the protein) to be generally down-regulated. The affected proteins were principally chaperones and cytoskeletal. In marked contrast, amphotericin B induced a more dramatic response, with 33/488 spots (9.5% of the total protein) generally up-regulated. The proteins were mostly involved in chaperoning and protein turnover. Combining antibiotics and amphotericin B had little overall effect, with only one (unidentified) protein being up-regulated. As this study identifies differential protein expression attributable to antibiotics/antimycotics, we urge caution when comparing and interpreting proteomic results from different laboratories where antibiotics/antimycotics have been used. We conclude that as antibiotics and antimycotics alter the proteome of cultured cells in markedly different ways their use should be avoided where possible.
机译：细胞培养被广泛用于研究基因或蛋白质对实验条件的响应。此类实验的价值取决于严格控制和对体外环境的了解。尽管有充分的文献记载描述了在临床环境中的毒性作用，但抗生素和抗真菌药仍常规用于细胞培养中，而不考虑其潜在毒性。我们在存在/不存在抗生素（青霉素/链霉素）和/或抗真菌两性霉素B的情况下培养MCF-7乳腺癌细胞。使用2D-DIGE和MALDI-MS / MS评估差异蛋白表达。抗生素通常导致8/488点（占蛋白质的1.3％）下调。受影响的蛋白质主要是伴侣蛋白和细胞骨架。与之形成鲜明对比的是，两性霉素B诱导了更剧烈的反应，通常上调了33/488个斑点（占总蛋白质的9.5％）。蛋白质主要参与伴侣和蛋白质更新。抗生素和两性霉素B的结合几乎没有总体效果，只有一种（未鉴定的）蛋白被上调。由于这项研究确定了可归因于抗生素/抗真菌药的蛋白质差异表达，因此在比较和解释使用了抗生素/抗真菌药的不同实验室的蛋白质组学结果时，我们敦促谨慎行事。我们得出的结论是，由于抗生素和抗真菌药会以明显不同的方式改变培养细胞的蛋白质组，因此应尽可能避免使用它们。

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