...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular biochemistry. >Subproteomic analysis of the mitochondrial proteins in rats 24 h after partial hepatectomy.
【24h】

Subproteomic analysis of the mitochondrial proteins in rats 24 h after partial hepatectomy.

机译：肝部分切除后24 h大鼠线粒体蛋白的亚蛋白质组学分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A 70% partial hepatectomy (70%PHx) induces cell proliferation until the original mass of the liver is restored. Mitochondria are involved directly in the process of liver regeneration (LR); however, their role in the early phase of LR is not clear. In an attempt to identify mitochondrial proteins that are correlated with the early phase of LR, we obtained a mitochondrial fraction via Nycodenz(R) density gradient centrifugation and subcellular proteomic analysis was performed. The mitochondrial proteins were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Compared to the sham-operation control group, 3 proteins were up-regulated and 22 proteins were down-regulated at 24 h after 70%PHx. We identified 22 differentially expressed proteins that were associated with carbohydrate metabolism, lipid metabolism, the respiratory chain and oxidation-phosphorylation, biotransformation and other metabolic pathways. Prohibitin, a proliferation-regulating protein that was down-regulated at 24 h after PHx, was analyzed by applying RNAi (PHBi) with BRL-3A. This demonstrated a decreased mitochondrial membrane potential, implying a potential role in maintaining mitochondrial integrity. These results indicated that hepatic mitochondrial adaptations to LR after 70%PHx affect various cellular metabolic pathways, which advances our knowledge of the role of mitochondria in the early phase of LR.
机译：70％的部分肝切除术（70％PHx）会诱导细胞增殖，直到恢复肝脏的原始质量。线粒体直接参与肝再生（LR）过程;但是，它们在LR早期的作用尚不清楚。为了鉴定与LR早期相关的线粒体蛋白，我们通过Nycodenz（R）密度梯度离心获得了线粒体级分，并进行了亚细胞蛋白质组学分析。通过二维凝胶电泳分离线粒体蛋白，并通过质谱鉴定。与假手术对照组相比，在70％PHx后24小时，有3种蛋白被上调，有22种蛋白被下调。我们鉴定了22种差异表达的蛋白质，这些蛋白质与碳水化合物代谢，脂质代谢，呼吸链和氧化磷酸化，生物转化及其他代谢途径有关。通过将RNAi（PHBi）与BRL-3A结合使用，可对PHx后24小时下调的增殖抑制蛋白Prohibitin进行分析。这表明线粒体膜电位降低，这暗示着维持线粒体完整性的潜在作用。这些结果表明70％PHx后肝线粒体对LR的适应会影响各种细胞代谢途径，这提高了我们对线粒体在LR早期作用的认识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号