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首页> 外文期刊>Journal of cellular biochemistry. >Expression of iron-related proteins during infection by bovine herpes virus type-1.
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Expression of iron-related proteins during infection by bovine herpes virus type-1.

机译：1型牛疱疹病毒感染过程中铁相关蛋白的表达。

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摘要

Bovine herpesvirus 1 (BHV-1), a dsDNA animal virus, is an economically important pathogen of cattle and the aetiological agent of many types of disease. The efficient replication of a DNA virus is strictly dependent on iron since this metal plays a crucial role in the catalytic center of viral ribonucleotide reductase. Consequently, iron metabolism is an important area for virus/host interaction and a large body of evidence suggests that viral infection is potentially influenced by the iron status of the host. The aim of the present study was to address the effects of BHV-1 on iron metabolism in Madin-Darby bovine kidney (MDBK) cells at different times of post-infection. For this purpose, cell viability, iron regulatory proteins (IRPs) activity and levels, transferrin receptor 1 (TfR-1), ferritin expression and LIP were evaluated. Our data demonstrate that a productive BHV-1 infection in MDBK cells determines an overall decrease of IRPs RNA-binding activity without affecting their expression. As consequence of this modulation, an increased ferritin mRNA translation and a decreased TfR-1 mRNA translation were also observed. Moreover, the LIP level was decreased following viral infection. These results are consistent with the hypothesis that by reducing the iron up-take and by enhancing the sequestration of free iron, animal cells will limit the iron availability for virus proliferation. Therefore, the results presented herein support the view that iron metabolism could be critical for the interaction between DNA viruses, such as BHV-1, and mammalian cells. Delineation of the interplay among pathogen and host may provide new antimicrobial agents.
机译：牛疱疹病毒1（BHV-1）是一种dsDNA动物病毒，是牛的一种经济上重要的病原体，也是多种疾病的病原体。 DNA病毒的有效复制严格取决于铁，因为这种金属在病毒核糖核苷酸还原酶的催化中心起着至关重要的作用。因此，铁代谢是病毒/宿主相互作用的重要领域，大量证据表明病毒感染可能受宿主铁状态的影响。本研究的目的是在感染后的不同时间处理BHV-1对Madin-Darby牛肾（MDBK）细胞中铁代谢的影响。为此，评估了细胞活力，铁调节蛋白（IRP）活性和水平，转铁蛋白受体1（TfR-1），铁蛋白表达和LIP。我们的数据表明，在MDBK细胞中生产性BHV-1感染决定了IRP RNA结合活性的总体下降，而不会影响其表达。由于这种调节，还观察到铁蛋白mRNA翻译增加和TfR-1 mRNA翻译减少。此外，病毒感染后LIP水平下降。这些结果与以下假设相吻合：通过减少铁的吸收并增强游离铁的螯合，动物细胞将限制铁可用于病毒增殖。因此，本文提供的结果支持这样的观点，即铁代谢对于DNA病毒（例如BHV-1）与哺乳动物细胞之间的相互作用至关重要。病原体和宿主之间相互作用的描述可能会提供新的抗菌剂。

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