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首页> 外文期刊>Journal of cellular biochemistry. >The nuclear actin-related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization.
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The nuclear actin-related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization.

机译：核肌动蛋白相关蛋白Act3p / Arp4影响酵母细胞形状和大量染色质组织。

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摘要

ACT3/ARP4 is an essential gene, coding for the actin-related protein Act3p/Arp4 of Saccharomyces cerevisiae located within the nucleus. Act3p/Arp4 is a stoichiometric component of the NuA4, INO80, and SWR1 chromatin modulating complexes, and recruits these complexes onto chromatin for their proper chromatin functions. Mutated Act3p/Arp4 leads to impairment of the functions of these complexes and affects transcription of specific genes. Our results revealed significant disorder in the cell size and shape of act3/arp4 mutant cells, when grown at permissive temperature. act3/arp4 mutants have also demonstrated an increase in their nuclear diameters, thus suggesting that Act3p/Arp4 is a key regulator in the maintenance of cellular shape and nuclear organization. Furthermore, the use of Chromatin Yeast Comet Assay (ChYCA) for assessment of single-cell bulk chromatin organization in act3/arp4 mutant cells allowed us to detect an elevated sensitivity toward nuclease action, denoting differences in higher-order chromatin structure of the mutants.
机译：ACT3 / ARP4是必需基因，编码位于细胞核内的酿酒酵母的肌动蛋白相关蛋白Act3p / Arp4。 Act3p / Arp4是NuA4，INO80和SWR1染色质调节复合物的化学计量组成部分，并将这些复合物募集到染色质上以发挥其适当的染色质功能。突变的Act3p / Arp4导致这些复合物功能受损，并影响特定基因的转录。我们的结果表明，在允许的温度下生长时，act3 / arp4突变细胞的细胞大小和形状存在明显的紊乱。 act3 / arp4突变体也已证明其核直径增加，因此表明Act3p / Arp4是维持细胞形状和核组织的关键调节剂。此外，使用染色质酵母彗星试验（ChYCA）评估act3 / arp4突变细胞中单细胞大量染色质的组织，使我们能够检测到对核酸酶作用的敏感性提高，这表明该突变体的高级染色质结构存在差异。

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