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首页> 外文期刊>Journal of cellular biochemistry. >Radiation-induced bystander effects in malignant trophoblast cells are independent from gap junctional communication.
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Radiation-induced bystander effects in malignant trophoblast cells are independent from gap junctional communication.

机译：辐射诱导的恶性滋养细胞细胞旁观者效应独立于间隙连接通讯。

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摘要

It is controversially discussed that irradiation induces bystander effects via gap junction channels and/or diffusible cellular factors such as nitric oxide or cytokines excreted from the cells into the environment. But up to now the molecular mechanism leading to a bystander response is not well understood. To discriminate between both mechanisms of bystander response, (i) mediated by gap junctional communication and/or (ii) mediated by diffusible molecules, we used non-communicating Jeg3 malignant trophoblast cells transfected with inducible gap junction proteins, connexin43 and connexin26, respectively, based on the Tet-On system. We co-cultivated X-ray irradiated and non-irradiated bystander Jeg3 cells for 4 h, separated both cell populations by flow cytometry and evaluated the expression of activated p53 by Western blot analysis. The experimental design was proven with communicating versus non-communicating Jeg3 cells. Interestingly, our results revealed a bystander effect which was independent from gap junctional communication properties and the connexin isoform expressed. Therefore, it seems more likely that the bystander effect is not mediated via gap junction channels but rather by paracrine mechanisms via excreted molecules in Jeg3 cells.
机译：有争议的讨论是辐射通过间隙连接通道和/或可扩散的细胞因子（如一氧化氮或从细胞排泄到环境中的细胞因子）诱导旁观者效应。但是到目前为止，导致旁观者反应的分子机制还不十分清楚。为了区分两种旁观者反应机制，（i）由间隙连接通讯介导的和/或（ii）由可扩散分子介导的，我们分别使用转染了可诱导间隙连接蛋白，连接蛋白43和连接蛋白26的非通信Jeg3恶性滋养层细胞，基于Tet-On系统。我们共培养了X射线照射的和未照射的旁观者Jeg3细胞4 h，通过流式细胞仪分离了两个细胞群，并通过Western blot分析评估了激活的p53的表达。通过通信与非通信Jeg3单元证明了实验设计。有趣的是，我们的结果显示了旁观者效应，其独立于间隙连接通讯特性和所表达的连接蛋白同工型。因此，似乎更可能不是通过间隙连接通道介导旁观者效应，而是通过Jeg3细胞中的排泄分子通过旁分泌机制介导。

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