...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular biochemistry. >p21 activated kinase 5 activates Raf-1 and targets it to mitochondria.
【24h】

p21 activated kinase 5 activates Raf-1 and targets it to mitochondria.

机译：p21激活的激酶5激活Raf-1并将其靶向线粒体。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Raf-1 is an important effector of Ras mediated signaling and is a critical regulator of the ERK/MAPK pathway. Raf-1 activation is controlled in part by phosphorylation on multiple residues, including an obligate phosphorylation site at serine 338. Previously PAK1 and casein kinase II have been implicated as serine 338 kinases. To identify novel kinases that phosphorylate this site, we tested the ability of group II PAKs (PAKs 4-6) to control serine 338 phosphorylation. We observed that all group II PAKs were efficient serine 338 kinases, although only PAK1 and PAK5 significantly stimulated Raf-1 kinase activity. We also showed that PAK5 forms a tight complex with Raf-1 in the cell, but not A-Raf or B-Raf. Importantly, we also demonstrated that the association of Raf-1 with PAK5 targets a subpopulation of Raf-1 to mitochondria. These data indicate that PAK5 is a potent regulator of Raf-1 activity and may control Raf-1 dependent signaling at mitochondria.
机译：Raf-1是Ras介导的信号传导的重要效应子，并且是ERK / MAPK途径的关键调节剂。 Raf-1激活部分受多个残基的磷酸化控制，包括丝氨酸338的专一磷酸化位点。以前，PAK1和酪蛋白激酶II被认为是丝氨酸338激酶。为了鉴定使该位点磷酸化的新型激酶，我们测试了II组PAK（PAK 4-6）控制丝氨酸338磷酸化的能力。我们观察到，尽管只有PAK1和PAK5能够显着刺激Raf-1激酶活性，但所有II组PAK都是有效的338丝氨酸激酶。我们还显示，PAK5在细胞中与Raf-1形成紧密的复合物，但在A-Raf或B-Raf中不形成。重要的是，我们还证明了Raf-1与PAK5的结合将Raf-1的亚群靶向线粒体。这些数据表明PAK5是Raf-1活性的有效调节剂，并可能控制线粒体上依赖Raf-1的信号传导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号