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首页> 外文期刊>Journal of cellular biochemistry. >Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.
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Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.

机译：胚胎未分化细胞在固定有E-钙粘蛋白的表面上显示散射活性。

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摘要

Rearrangement of cell-cell adhesion is a critical event in embryonic development and tissue formation. We investigated the regulatory function of E-cadherin, a key adhesion protein, in the developmental process by using E-cadherin/IgG Fc fusion protein as an adhesion matrix in cell culture. F9 embryonal carcinoma cells usually form colonies when cultured on gelatin or fibronectin matrices. However, F9 cells cultured on the E-cadherin/IgG Fc fusion protein matrix formed a scattered distribution, with a different cytoskeletal organization and E-cadherin-rich protrusions that were regulated by Rac1 activity. The same scattering activity was observed in P19 embryonal carcinoma cells. In contrast, three types of differentiated cells, NMuMG mammary gland cells, MDCK kidney epithelial cells, and mouse primary isolated hepatocytes, did not show the scattering activity observed in F9 and P19 cells. These results suggest that migratory behavior on an E-cadherin-immobilized surface is only observed in embryonic cells, and that the regulatory mechanisms underlying E-cadherin-mediated cell adhesion vary with the state of differentiation.
机译：细胞-细胞粘附的重排是胚胎发育和组织形成中的关键事件。我们通过在细胞培养中使用E-cadherin / IgG Fc融合蛋白作为粘附基质，研究了关键粘附蛋白E-cadherin在发育过程中的调控功能。当在明胶或纤连蛋白基质上培养时，F9胚胎癌细胞通常形成集落。但是，在E-cadherin / IgG Fc融合蛋白基质上培养的F9细胞形成了分散的分布，具有不同的细胞骨架组织和受Rac1活性调节的E-cadherin丰富的突起。在P19胚胎癌细胞中观察到相同的散射活性。相反，三种类型的分化细胞，NMuMG乳腺细胞，MDCK肾上皮细胞和小鼠原代分离的肝细胞，未显示在F9和P19细胞中观察到的散射活性。这些结果表明，仅在胚胎细胞中观察到固定有E-钙粘蛋白的表面上的迁移行为，并且E-钙粘蛋白介导的细胞粘附的调节机制随分化状态而变化。

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