BACKGROUND AND OBJECTIVES: We explored the relationship between the mutation pattern of Ki-ras and the clinicopathological features of colorectal cancers (CRCs). METHODS: Relationships between clinicopathological parameters and Ki-ras mutation status were analyzed in 255 CRC patients using the chi-square and student t-tests. Kaplan-Meier survival curves were compared using the log-rank test. RESULTS: Ki-ras mutation occurred in 43.9% of tumors, and 83% affected codon 12. The most frequent mutations were GGT->GAT (Gly->Asp) (37.5%), followed by GGT->GTT (Gly->Val) (31.3%), both in codon 12. The frequency of Ki-ras mutation was similar for different tumor stages (38.2-47.8%). The mucin component of tumors was significantly associated with Ki-ras mutation. The 4-year overall and disease-free survival was 61% and 54%, respectively, for patients with Ki-ras mutated tumors, and 73% and 60% for patients with nonmutated tumors (not statistically significant). Patients with Ki-ras mutated tumors had lower plasma folate (24 ng/dl) than those bearing nonmutated tumors (37 ng/dl). Patients with G->T Ki-ras mutations had the lowest folate level (22 ng/dl), followed by those with G->A mutations (25 ng/dl). CONCLUSIONS: Ki-ras mutated colorectal tumors have a higher mucin production and higher differentiation, and are associated with lower plasma folate levels and a relatively poorer disease outcome.
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机译：背景与目的：我们探讨了Ki-ras基因突变模式与结直肠癌（CRC）临床病理特征之间的关系。方法：使用卡方检验和学生t检验分析了255例CRC患者的临床病理参数与Ki-ras突变状态之间的关系。使用对数秩检验比较Kaplan-Meier生存曲线。结果：Ki-ras突变发生在43.9％的肿瘤中，83％的密码子受到影响。最常见的突变是GGT-> GAT（Gly-> Asp）（37.5％），其次是GGT-> GTT（Gly-> Val（31.3％），均位于密码子12中。不同肿瘤分期的Ki-ras突变频率相似（38.2-47.8％）。肿瘤的粘蛋白成分与Ki-ras突变显着相关。 Ki-ras突变肿瘤患者的4年总体生存率和无病生存率分别为61％和54％，非突变肿瘤患者的4年总体生存率和无病生存率分别为73％和60％（无统计学意义）。 Ki-ras突变肿瘤患者的血浆叶酸（24 ng / dl）比未突变肿瘤患者（37 ng / dl）低。 G-> T Ki-ras突变的患者叶酸水平最低（22 ng / dl），其次是G-> A突变的患者（25 ng / dl）。结论：Ki-ras突变的结直肠肿瘤具有更高的粘蛋白产生和更高的分化，并与较低的血浆叶酸水平和相对较差的疾病结果有关。
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