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首页> 外文期刊>Journal of Surgical Oncology >Free peritoneal tumor cells detection in gastric and colorectal cancer patients
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Free peritoneal tumor cells detection in gastric and colorectal cancer patients

机译：胃癌和大肠癌患者腹膜游离肿瘤细胞的检测

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Background: Free peritoneal tumor cells (FPTC) derive from the detachment of primary cancer and may result in peritoneal carcinomatosis. Since peritoneal lavage cytology has low sensitivity in detecting FPTC, our aim was to estimate the clinical relevance of FPTC detected using an approach based on multiple molecular techniques. Materials and Methods: Samples of peritoneal lavage were collected from 27 gastric and 48 colorectal cancer patients. FPTC recovery and detection from peritoneal washes was performed by cytological examination and immunomagnetic enrichment for epithelial cells followed by immunofluorescence analysis for epithelial marker EpCAM/CD326 and carcinoembryonic antigen (CEA). CEA and CK20 mRNA levels were quantified using a real-time qRT-PCR system. Results: For gastric carcinoma the FPTC positivity rate acquired by cytology, immunofluorescence and qRT-PCR was 14.8%, 14.8%, and 78% and for colorectal carcinoma was 0%, 17%, and 42%, respectively. qRT-PCR positivity was correlated with a poor cancer-specific survival and time-to-recurrence rates in both gastric and colorectal carcinoma. Conclusions: Epithelial immunoenrichment and immunofluorescence analysis allows unequivocal identification of the FPTC. The real time qRT-PCR showed higher sensitivity for the detection of CEA and CK20 mRNA levels and confirmed its prognostic value in gastrointestinal cancers.
机译：背景：游离腹膜肿瘤细胞（FPTC）源自原发性癌的脱离，并可能导致腹膜癌变。由于腹腔灌洗细胞学检测FPTC的敏感性较低，因此我们的目的是评估使用基于多种分子技术的方法检测到的FPTC的临床相关性。材料与方法：收集27例胃癌和48例大肠癌患者的腹腔灌洗样本。通过对上皮细胞进行细胞学检查和免疫磁富集，然后对上皮标记EpCAM / CD326和癌胚抗原（CEA）进行免疫荧光分析，从腹膜清洗液中恢复和检测FPTC。使用实时qRT-PCR系统定量CEA和CK20 mRNA水平。结果：对于胃癌，通过细胞学，免疫荧光和qRT-PCR获得的FPTC阳性率分别为14.8％，14.8％和78％，对于结直肠癌，其FPTC阳性率分别为0％，17％和42％。在胃癌和结肠直肠癌中，qRT-PCR阳性与不良的癌症特异性生存率和复发时间相关。结论：上皮免疫富集和免疫荧光分析可以明确鉴定FPTC。实时qRT-PCR显示出对CEA和CK20 mRNA水平检测的更高敏感性，并证实了其在胃肠道癌症中的预后价值。

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