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首页> 外文期刊>Clinical and Experimental Immunology: An Official Journal of the British Society for Immunology >Anti-beta1-adrenergic receptor autoantibodies in patients with chronic Chagas heart disease.
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Anti-beta1-adrenergic receptor autoantibodies in patients with chronic Chagas heart disease.

机译：慢性Chagas心脏病患者的抗β1肾上腺素能受体自身抗体。

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摘要

Chronic Chagas heart disease (cChHD), a chronic manifestation of the Trypanosoma cruzi infection, is characterized by high antibody levels against the C-terminal region of the ribosomal P proteins (i.e. peptide R13, EEEDDDMGFGLFD) which bears similarity with the second extracellular loop of beta1-adrenergic receptor (beta1-AR, peptide H26R HWWRAESDEARRCYNDPKCCDFVTNR). Because it has not been demonstrated clearly that IgGs from cChHD patients bind to native human beta1-AR, the aim of this study was to investigate further the physical interaction between cChHD IgGs and the human beta1-AR. Immunofluorescence assays demonstrated the binding of these antibodies to the receptor expressed on stably transfected cells, together with a beta1-AR agonist-like effect. In addition, immunoadsorption of the serum samples from cChHD patients with a commercially available matrix, containing peptides representing the first and the second extracellular loop of the beta1-AR, completely abolished reactivity against the H26Rpeptide and the physiological response to the receptor. The follow-up of this specificity after in vitro immunoadsorption procedures suggests that this treatment might be used to diminish significantly the serum levels of anti-beta1-AR antibodies in patients with Chagas heart disease.
机译：慢性Chagas心脏病（cChHD）是克鲁斯氏锥虫感染的一种慢性表现，其特征是针对核糖体P蛋白（即肽R13，EEEDDDMGFGLFD）的C端区域的抗体水平高，该抗体水平与C. beta1-肾上腺素能受体（beta1-AR，肽H26R HWWRAESDEARRCYNDPKCCDFVTNR）。由于尚未清楚证明cChHD患者的IgG与天然人beta1-AR结合，因此本研究的目的是进一步研究cChHD IgG与人beta1-AR之间的物理相互作用。免疫荧光分析表明，这些抗体与稳定转染的细胞上表达的受体结合，并具有类似β1-AR激动剂的作用。此外，使用市售基质（包含代表β1-AR第一个和第二个细胞外环的肽）对cChHD患者的血清样品进行免疫吸附，完全消除了对H26R肽的反应性和对受体的生理反应。在体外免疫吸附程序之后对该特异性的随访表明，该治疗方法可用于显着降低Chagas心脏病患者的抗β1-AR抗体血清水平。

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