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首页> 外文期刊>魚病研究 >Prevention of Flavobacterium psychrophilum Vertical Transmission by lodophor Treatment of UnfertilizedEggs in Salmonids
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Prevention of Flavobacterium psychrophilum Vertical Transmission by lodophor Treatment of UnfertilizedEggs in Salmonids

机译：碘伏处理鲑鱼未受精卵预防精神病黄杆菌垂直传播

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摘要

We evaluated the efficacy of povidone-iodine treatments in preventing vertical transmission of Flavobacterium psychrophilum in the eggs of masu salmon Oncorhynchus masou, Japanese char Salvelinus pluvius, coho salmon O. kisutch and rainbow trout O. mykiss. When newly spawned eggs were immersion-challenged with F. psychrophilum (>10~9 CFU/mL) and subsequently disinfected with povidone-iodine (50 ppm in PBS,15 min) prior to fertilization, the pathogen was not detected in the contents of the eggs. Without this pre-fertilization disinfection with povidone-iodine, however, procedures such as water-hardening of immersion-challenged eggs in povidone-iodine (50-100 ppm in water) and rinsing of the F. psychrophilum-couXam'maXed eggs with PBS were insufficient to control the intra-ovum infection. In a separate experiment using coho salmon eggs challenged with F. psychrophilum, pre-fertilization disinfection was effective to prevent bacterial cold-water disease in the produced fry. Surface disinfection of unfertilized eggs immediately after spawning is thus essential to prevent vertical transmission of F. psychrophilum.
机译：我们评估了聚乙烯吡咯烷酮碘处理在预防鲑鱼Oncorhynchus masou，日本char salvelinus pluvius，银大麻哈鱼O. kisutch和虹鳟鱼O. mykiss的卵中嗜冷黄杆菌垂直传播的功效。当刚产卵的鸡蛋用嗜冷链霉菌（> 10〜9 CFU / mL）浸没，然后在受精前用聚维酮碘（PBS中50 ppm，15分钟）消毒时，在细菌中未检出病原体。鸡蛋。但是，如果没有用聚维酮碘进行这种预施肥消毒，则需要进行程序处理，例如将浸没挑战的卵在聚维酮碘中进行水硬化（水中50-100 ppm），并用PBS冲洗嗜冷链霉菌卵。不足以控制卵内感染。在一个单独的实验中，该实验使用了用拟南芥（F. psychrophilum）攻击的银大麻哈鱼卵，受精前的消毒可有效预防所生产鱼苗中的细菌性冷水病。因此，产卵后立即对未受精卵进行表面消毒对于防止嗜热链球菌的垂直传播至关重要。

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