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首页> 外文期刊>魚病研究 >TaqMan Real-Time PCR Detection and Phylogenetic Analysis of Cryptocaryon irritans in Nomi Bay, Kochi, Japan
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TaqMan Real-Time PCR Detection and Phylogenetic Analysis of Cryptocaryon irritans in Nomi Bay, Kochi, Japan

机译：TaqMan实时PCR检测和日本高知能美湾隐孢子虫的系统发育分析

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摘要

The 5.8S rRNA gene of C. irritans in water and mud substrate samples were quantified at 6 or 7 stations in Nomi Bay, Kochi, Japan twice a month from the late June to the middle December, using a TaqMan real-time PCR assay developed in the present study. The number of copies of the gene in water peaked at 4:00 am over a 20-h sampling period in October. The mean numbers of the gene copies equivalent to 4.465-6,590 theronts/L were detected from water sampled at a station close to cages containing great amberjack Seriola dumerili affected with cryptocaryoniasis in early October and late November, when cryptocaryoniasis outbroke. The frequency of detection of the gene in the substrate mud was highest at a station far from the affected area, suggesting that the distribution of tomonts of C. irritans were influenced with tidal currents. The 18S-ITS1 region of C. irritans was PCR-amplified from a water sample, sequenced and compared with the sequences previously obtained from the parasite of various localities. The present sequence was contained in a Glade mainly comprising the sequences obtained in China, suggesting that the greater amberjack juveniles imported from southern China was a source of C. irritans in Nomi Bay.
机译：使用开发的TaqMan实时PCR分析方法，从6月下旬至12月中旬，每月两次在日本高知市Nomi湾的6或7个站定量分析水和泥底物样品中的ir。ir。5.8S rRNA基因。在目前的研究中。在十月份的20小时采样期内，水中的基因拷贝数达到了凌晨4:00。在10月初和11月下旬爆发隐性念珠菌病时，从靠近盛有大型犬的Seriola dumerili的网箱附近的水站采样到的水样中检测到的基因拷贝平均数为4.465-6,590 ron / L。在底泥中检测到该基因的频率在远离受灾地区的某个位置最高，这表明潮汐影响了C. irritans tomont的分布。从水样中PCR扩增出C. irritans的18S-ITS1区，进行测序，并与先前从各个地方的寄生虫获得的序列进行比较。目前的序列包含在Glade中，该Glade主要包含在中国获得的序列，这表明从中国南部进口的较大的mber鱼是Nomi湾的irirans的来源。

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