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首页> 外文期刊>魚病研究 >Identification and Pathogenicity of Intracellular Francisella Bacterium in Three-line Grunt Parapristipoma trilineatum
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Identification and Pathogenicity of Intracellular Francisella Bacterium in Three-line Grunt Parapristipoma trilineatum

机译：三系咕unt声副线菌中细胞内弗氏杆菌的鉴定和致病性

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摘要

Bacteriological examination was conducted on the disease of cultured marine fish, three-line grunt Parapristipoma trilineatum (Japanese common name: isaki). The affected fish characteristically showed white spots of granulomas containing an intracellular bacterium in the kidney and spleen. Total DMA was extracted from the kidney of the diseased fish. The coding region of small subunit ribosomal RNA (16S rDNA) of eubacteria was amplified from the DNA by PCR, and nucleotide sequence was determined. Thesequenced 16S rDNA revealed high similarity (97.3-98.5%) to those of Francisella species. On the basis of phylogenetic analysis, the closest relative organism was revealed to be Francisella philomiragia. A bacterium was isolated from the spleen of one affected fish using cystine heart agar medium with 1% hemoglobin. The 16S rDNA sequence of the isolated bacterium was consistent with that determined from the kidney tissue of the affected fish. An experimental infection with the isolate exhibited the same disease signs in the injected fish, and the bacterium was reisolated from these fish. These results demonstrate that the intracellular bacterium is pathogenic and is identified to the genus Francisella. This is the first report of pathogenic Francisella from marine fish.
机译：对养殖的海水鱼三线gr三叉线虫（日本俗名：isaki）的病原进行了细菌学检查。受影响的鱼的特征是在肾脏和脾脏中出现肉芽肿的白色斑点，肉芽肿中含有细胞内细菌。总DMA是从患病鱼类的肾脏中提取的。通过PCR从DNA中扩增出真细菌的小亚单位核糖体RNA（16S rDNA）的编码区，并确定了核苷酸序列。序列化的16S rDNA与弗朗西斯菌属物种具有高度相似性（97.3-98.5％）。根据系统发育分析，最接近的相对生物是费氏弗朗西斯菌。使用含有1％血红蛋白的胱氨酸琼脂培养基从一只患鱼的脾脏中分离出细菌。分离细菌的16S rDNA序列与从患鱼的肾脏组织确定的序列一致。用分离物进行的实验性感染在注入的鱼中表现出相同的疾病征兆，并且从这些鱼中分离出细菌。这些结果证明细胞内细菌是致病的，并且被鉴定为弗朗西斯菌属。这是海水鱼类致病性弗朗西斯菌的首次报道。

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