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首页> 外文期刊>魚病研究 >Development of the Macronucleus of Cryptocaryon irritans, a Parasitic Ciliate of Marine Teleosts, and its Ingestion and Digestion of Host Cells
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Development of the Macronucleus of Cryptocaryon irritans, a Parasitic Ciliate of Marine Teleosts, and its Ingestion and Digestion of Host Cells

机译：硬骨鱼的寄生虫纤毛虫隐核虫的大核的发育及其对宿主细胞的摄取和消化

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摘要

We investigated the development of the macronucleus of Cryptocaryon irritans, and the ingestion and digestion of host cells by the parasite. All developmental stages of the parasite except the theront (trophont, protomont and tomont) were examined with histological staining and/or whole mount staining. The sections were also subjected to in situ hybridization targeting the 18S rRNA gene of the host fish. The macronucleus developed negligibly, maintaining its four-segmented shape in trophonts, elongated and formed a massive nucleus in tomonts before cell division, and subsequently underwent repeated divisions to generate theronts. Denatured host cells, examined in situ hybridization, were accumulated in trophonts, and disappeared in tomonts by the beginning of cell division. Denatured host cells were also observed in the host tissue surrounding trophonts. They had condensed nuclei. Protomonts filled with denatured host cells presented a 180-bp DNA ladder in gel electrophoresis, suggesting apoptosis of the host cells. These results indicate that DNA synthesis occurs exclusively in the early stage of the tomont and that host cells are fed and accumulated, probably as apoptotic cells, in the trophont and are digested in the early tomont stage.
机译：我们调查了隐孢子虫大核的发育，以及寄生虫对宿主细胞的摄取和消化。用组织学染色和/或整装染色检查除the虫以外的所有寄生虫的发育阶段（tro 、,和to）。还对切片进行靶向宿主鱼的18S rRNA基因的原位杂交。大细胞核的发育可以忽略不计，在小节中保持其四段形状，在细胞分裂前在托蒙斯中伸长并形成了巨大的细胞核，随后进行了多次分裂以产生火星。变性的宿主细胞经过原位杂交后，在细胞中积累，并在细胞分裂开始时消失在托蒙山脉。还可以在Trophons周围的宿主组织中观察到变性的宿主细胞。他们凝聚了原子核。充满变性宿主细胞的Protomonts在凝胶电泳中呈现出180 bp的DNA阶梯，提示了宿主细胞的凋亡。这些结果表明，DNA合成仅发生在托蒙特的早期，并且宿主细胞被填充和积累，可能是凋亡细胞，在套管针中并在托蒙特的早期被消化。

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