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首页> 外文期刊>植物防疫所調查研究報告 >Studies on the diagnosis of foreign bacterial diseases of quarantine significance VII. preparation of selective medium and antiserum for the detection of Clavibacter michiganensis subsp. nebraskensis.
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Studies on the diagnosis of foreign bacterial diseases of quarantine significance VII. preparation of selective medium and antiserum for the detection of Clavibacter michiganensis subsp. nebraskensis.

机译：对外来细菌性疾病的检疫诊断意义研究七。密歇根氏杆菌亚种检测的选择性培养基和抗血清的制备内布拉斯加州。

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A selective medium (NSM) was developed for isolating Clavibacter michiganensis subsp. nebraskensis, the causal agent of Goss's wilt, which is seedborne and of no occurrence in Japan. Also, antiserum was prepared for diagnosis of it. On NSM, Cl. m. subsp. nebraskensis formed capitated, smooth and salmon-pink colonies after 3-4 days incubation at 30鳦, and blackening of the medium around the colonies was observed. Depending on this colony characteristic, Cl. m. subsp. nebraskensis was easily distinguished from other bacteria such as Cl. m. subsp. michiganensis, Curtobacterium flaccumfaciens pv. flaccumfaciens, Rhodococcus fascians etc., which could grow on NSM. The colony forming efficiency on NSM was 72-177%. In gel-double-diffusion test and slide-agglutination test using the antiserum against this bacterium and various antigen of the other bacteria, it suggested that there was a relatively antigenic homogeneity among all 13 strains of Cl. m. subsp. nebraskensis. The antiserum absorbed with the antigen of Cu. fl. pv. flaccumfaciens, which had a weak reaction with crude antiserum, had become more specific on subspecies level. Therefore, NSM and the antiserum are useful for diagnosis of the disease caused by Cl. m. subsp. nebraskensis.
机译：开发了一种选择性培养基（NSM），用于分离密歇根杆状杆菌亚种。 nebraskensis，高斯枯萎病的病原体，是种子传播的，在日本没有发生。另外，准备了抗血清用于诊断。在NSM上，Cl。米亚种内布拉斯加州在30°C孵育3-4天后形成了头状，光滑和鲑鱼粉红色的菌落，并且观察到菌落周围的培养基变黑。取决于该菌落特征，Cl。米亚种内布拉斯加州很容易与其他细菌（如Cl）区分开。米亚种密歇根州曲霉杆菌PV。 flaccumfaciens，Rhodococcus fascians等，它们可以在NSM上生长。 NSM上菌落形成效率为72-177％。在使用针对该细菌和其他细菌的各种抗原的抗血清的凝胶双扩散试验和载玻片凝集试验中，表明在所有13个C1菌株中都具有相对抗原的同质性。米亚种内布拉斯加州。抗血清被铜的抗原吸收。 fl。 pv。与粗制抗血清反应较弱的黄曲霉在亚种水平上变得更加特异性。因此，NSM和抗血清对于诊断由Cl引起的疾病是有用的。米亚种内布拉斯加州。

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