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首页> 外文期刊>Biopreservation and biobanking >The Replacement of Monosaccharide by Mannitol or Sorbitol in the Freezing Extender Enhances Cryosurvival of Ram Spermatozoa
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The Replacement of Monosaccharide by Mannitol or Sorbitol in the Freezing Extender Enhances Cryosurvival of Ram Spermatozoa

机译：冷冻扩展剂中甘露醇或山梨糖醇替代单糖可增强Ram精子的低温存活。

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In this study, the protective effects of monosaccharides (glucose and fructose) and sugar alcohols (mannitol, sorbitol, and xylitol) on frozen ram spermatozoa were evaluated and compared. The motility, moving velocity, and hypoosmotic swelling capability of spermatozoa frozen with monosaccharide or sugar alcohol were measured using a computer-assisted spermatozoa analyzer system. The acrosome status, membrane integrity, distribution of phosphatidylserine (PS), and mitochondrial membrane potential (MMP) were analyzed using fluorescence staining and flow cytometry. The results indicated that similar to glucose or fructose, the presence of sugar alcohol in the freezing extender cannot significantly improve the motility and moving velocity of ram spermatozoa equilibrated at 5 degrees C. In terms of motility, pathway velocity, curve velocity, hypoosmotic swelling capability, acrosome and membrane integrity, and MMP, the inclusion of mannitol or sorbitol in the extender can significantly improve the quality of frozen-thawed ram spermatozoa compared to glucose or fructose. However, the effects of mannitol or sorbitol on linear velocity and PS distribution of frozen-thawed spermatozoa were similar to those of the monosaccharides (p>0.05). In addition, the ability of xylitol to protect acrosome and maintain MMP in frozen-thawed spermatozoa was significantly higher compared with glucose or fructose (p<0.05), although it could not improve the other evaluated parameters. Finally, there is no significant difference existing between mannitol and sorbitol with respect to the above evaluated parameters. In conclusion, the replacement of glucose or fructose by mannitol or sorbitol in a freezing extender can improve the postthaw quality of ram spermatozoa under specific freezing conditions. Moreover, the protective effects of mannitol and sorbitol on frozen-thawed ram spermatozoa are superior to that of xylitol. However, in the presence of sugar alcohols, the cryoinjury on spermatozoa membrane is still serious. In the future, the question of protecting the membrane of frozen-thawed spermatozoa needs further research.
机译：在这项研究中，评估并比较了单糖（葡萄糖和果糖）和糖醇（甘露醇，山梨糖醇和木糖醇）对冷冻公羊精子的保护作用。使用计算机辅助精子分析仪系统测量用单糖或糖醇冷冻的精子的运动性，运动速度和低渗溶胀能力。使用荧光染色和流式细胞仪分析顶体状态，膜完整性，磷脂酰丝氨酸（PS）的分布和线粒体膜电位（MMP）。结果表明，与葡萄糖或果糖相似，冷冻增量剂中糖醇的存在不能显着提高平衡在5摄氏度的ram精子的运动性和运动速度。就运动性，路径速度，曲线速度，低渗溶胀能力而言，顶体和膜的完整性以及MMP，与葡萄糖或果糖相比，在增量剂中加入甘露醇或山梨糖醇可显着提高冷冻融化的Ram精子的质量。然而，甘露醇或山梨糖醇对冻融精子的线速度和PS分布的影响与单糖相似（p> 0.05）。另外，与葡萄糖或果糖相比，木糖醇保护冻融的精​​子中的顶体和维持MMP的能力明显高于葡萄糖或果糖（p <0.05），尽管它不能改善其他评估参数。最后，就上述评估参数而言，甘露醇和山梨醇之间不存在显着差异。总之，在冷冻增量剂中用甘露醇或山梨糖醇替代葡萄糖或果糖可以改善在特定冷冻条件下精子的融化后质量。此外，甘露醇和山梨糖醇对冻融的公羊精子的保护作用优于木糖醇。然而，在糖醇的存在下，精子膜上的冷冻损伤仍然很严重。将来，保护冻融精子膜的问题需要进一步研究。

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