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首页> 外文期刊>Biopreservation and biobanking >Impact of Whole-Blood Processing Conditions on Plasma and Serum Concentrations of Cytokines
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Impact of Whole-Blood Processing Conditions on Plasma and Serum Concentrations of Cytokines

机译：全血处理条件对细胞因子血浆和血清浓度的影响

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Pre-analytical variations in plasma and serum samples can occur because of variability in whole-blood processing procedures. The aim of this study was to determine the impact of delayed separation of whole blood on the plasma and serum concentrations of cytokines. The concentrations of 16 cytokines were measured in plasma and serum samples when the centrifugation of whole blood at room temperature was delayed for 4, 6, 24, or 48h, and the values were compared with those observed after separation within 2h of whole-blood collection. Receiver operating characteristic (ROC) curve analysis was also performed for cytokines to determine whether cytokine levels in plasma and serum samples can be used to assess delayed separation of whole blood. Plasma concentrations of interleukin (IL)-1, granulocyte-macrophage colony-stimulating factor (GM-CSF), and soluble CD40 ligand (sCD40L) and serum concentrations of IL-1, IL-6, IL-8, macrophage inflammatory protein-1 (MIP-1), and MIP-1 increased significantly (>2-fold) when separation was delayed at room temperature for 24h. The concentrations of 6 of these cytokines (all except serum IL-1 and IL-6) demonstrated high diagnostic performance (area under the ROC curve >0.8) for delayed separation of whole blood. Furthermore, these cytokine concentrations typically exhibited high sensitivity and specificity at each optimal cutoff point. Conversely, IL-17A was stable in both plasma and serum samples, even when whole-blood centrifugation was delayed at room temperature for 48h. This study shows that certain cytokines (IL-1, GM-CSF, sCD40L, IL-8, MIP-1, and MIP-1) could be used for assessing the quality of plasma or serum samples.
机译：由于全血处理程序的差异，血浆和血清样品可能会发生分析前变化。这项研究的目的是确定全血延迟分离对血浆和血清中细胞因子浓度的影响。当全血在室温下延迟4、6、24或48h离心时，测量血浆和血清样品中16种细胞因子的浓度，并将其与全血收集后2h分离后观察到的值进行比较。 。还对细胞因子进行了接收者操作特征（ROC）曲线分析，以确定血浆和血清样品中的细胞因子水平是否可用于评估全血的延迟分离。血浆白细胞介素（IL）-1，粒细胞巨噬细胞集落刺激因子（GM-CSF）和可溶性CD40配体（sCD40L）的浓度以及血清IL-1，IL-6，IL-8，巨噬细胞炎性蛋白- 1（MIP-1），并且当分离在室温下延迟24h时，MIP-1显着增加（> 2倍）。这些细胞因子中的6种浓度（除血清IL-1和IL-6以外的所有浓度）表现出对全血延迟分离的高诊断性能（ROC曲线下面积> 0.8）。此外，这些细胞因子浓度通常在每个最佳临界点都表现出高灵敏度和特异性。相反，即使全血离心在室温下延迟48h，IL-17A在血浆和血清样品中也稳定。这项研究表明某些细胞因子（IL-1，GM-CSF，sCD40L，IL-8，MIP-1和MIP-1）可用于评估血浆或血清样品的质量。

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