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首页> 外文期刊>Журнал аналитической химии >Amperometric biosensor based on deoxyribonucleic acid and platinum(II) complexes: new analytical capabilities
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Amperometric biosensor based on deoxyribonucleic acid and platinum(II) complexes: new analytical capabilities

机译:基于脱氧核糖核酸和铂(II)配合物的安培生物传感器:新的分析能力

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摘要

A new method for the immobilization of DNA on a nitrocellulose membrane was proposed. The activity of immobilized biomolecules is retained their activity. The immobilized DNA was used as the biosensitive part of an amperometric sensor based on a stationary mercury-film electrode. To extend the analytical capabilities of the sensor, the peak current of catalytic hydrogen evolution at -1.4 V was examined (this peak current occurs in the complexation of platinum(II) with antibodies to DNA). The biosensor was used for determining antibodies to DNA in the concentration range 5.0 X 10~(-10) - 7.0 X 10~(-8) M. An immunoassay procedure was proposed.
机译:提出了一种将DNA固定在硝酸纤维素膜上的新方法。固定的生物分子的活性保持其活性。固定的DNA被用作基于固定汞膜电极的安培传感器的生物敏感部分。为了扩展传感器的分析能力,检查了在-1.4 V时催化氢释放的峰值电流(该峰值电流出现在铂(II)与抗DNA抗体的复合物中)。该生物传感器用于确定浓度范围为5.0 X 10〜(-10)-7.0 X 10〜(-8)M的DNA抗体。提出了一种免疫测定方法。

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