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首页> 外文期刊>ミルクサイエンス >Identification of lactic acid bacteria from raw milk of Wagyu cattle and their tolerance to simulated digestive juice
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Identification of lactic acid bacteria from raw milk of Wagyu cattle and their tolerance to simulated digestive juice

机译：和牛牛乳中乳酸菌的鉴定及其对模拟消化液的耐受性

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The purpose of this study is to investigate lactic acid bacterial distribution in raw milk of Wagyu cattle, and to evaluate a tolerance of the lactic acid bacteria against simulated digestive juices. Isolates from raw milk were identified as Lactobacillus reuteri, Lactobacillus plantarum, Lactococcus garvieae, Lactococcus lactis, Enterococcus hirae, Enterococcus faecalis, Enterococcus saccharolyticus, Weissella thailandensis and Pediococcus pentosaceus based on their physiological and biochemical characteristics as well as by16S rDNA sequence analysis. Lb. reuteri PUHM1004 and E. hirae PUHM1011 exhibited more than 50% of survival rate after 6 hours incubation in MRS broth supplemented with 0.3% oxgall. These strains survived after 3 hours in simulated gastric juice adjusted pH 2.0， and grew in a simulated intestinal juice. These strains could be used for probiotic food and feed.
机译：本研究的目的是调查和牛牛乳中乳酸菌的分布，并评估乳酸菌对模拟消化液的耐受性。原料奶的分离物通过生理和生化特征分析，通过DNA序列分析，鉴定出罗伊氏乳杆菌，植物乳杆菌，加尔乳杆菌，乳酸乳球菌，平肠肠球菌，粪肠球菌，解糖肠球菌，泰国魏氏菌和戊糖戊球菌r16。磅。在补充了0.3％oxgall的MRS肉汤中孵育6小时后，罗伊特氏菌PUHM1004和大肠埃希菌PUHM1011的存活率超过50％。这些菌株在pH 2.0的模拟胃液中存活3小时，并在模拟肠液中生长。这些菌株可用于益生菌食品和饲料。

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