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首页> 外文期刊>Biophysical Journal >Direct imaging of individual intrinsic hydration layers on lipid bilayers at Angstrom resolution.
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Direct imaging of individual intrinsic hydration layers on lipid bilayers at Angstrom resolution.

机译：在Angstrom分辨率下对脂质双层上的各个固有水合层进行直接成像。

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摘要

The interactions between water and biological molecules have the potential to influence the structure, dynamics, and function of biological systems, hence the importance of revealing the nature of these interactions in relation to the local biochemical environment. We have investigated the structuring of water at the interface of supported dipalmitoylphosphatidylcholine bilayers in the gel phase in phosphate buffer solution using frequency modulation atomic force microscopy (FM-AFM). We present experimental results supporting the existence of intrinsic (i.e., surface-induced) hydration layers adjacent to the bilayer. The force versus distance curves measured between the bilayer and the AFM tip show oscillatory force profiles with a peak spacing of 0.28 nm, indicative of the existence of up to two hydration layers next to the membrane surface. These oscillatory force profiles reveal the molecular-scale origin of the hydration force that has been observed between two apposing lipid bilayers. Furthermore,FM-AFM imaging at the water/lipid interface visualizes individual hydration layers in three dimensions, with molecular-scale corrugations corresponding to the lipid headgroups. The results demonstrate that the intrinsic hydration layers are stable enough to present multiple energy barriers to approaching nanoscale objects, such as proteins and solvated ions, and are expected to affect membrane permeability and transport.
机译：水与生物分子之间的相互作用可能会影响生物系统的结构，动力学和功能，因此，揭示与本地生化环境有关的这些相互作用的性质非常重要。我们已经使用调频原子力显微镜（FM-AFM）研究了磷酸盐缓冲溶液中凝胶相中支持的二棕榈酰磷脂酰胆碱双分子层界面水的结构。我们提出的实验结果支持了与双层相邻的内在（即表面诱导）水合层的存在。在双层和AFM尖端之间测得的力与距离的关系曲线显示出振荡力曲线，其峰间距为0.28 nm，这表明在膜表面附近最多存在两个水合层。这些振荡力曲线揭示了在两个相对的脂双层之间观察到的水合力的分子尺度起源。此外，在水/脂质界面的FM-AFM成像可以在三个维度上可视化各个水合层，并且分子级皱纹对应于脂质头基。结果表明，本征水合层足够稳定，可以对接近纳米级的物体（例如蛋白质和溶剂化离子）提供多种能垒，并有望影响膜的渗透性和运输。

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