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首页> 外文期刊>日本食品微生物学会雑誌 >Crabs and Japanese Mud shrimp by Viorio vulnificus and Serotyping and Drug Sensitivity of Isolates
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Crabs and Japanese Mud shrimp by Viorio vulnificus and Serotyping and Drug Sensitivity of Isolates

机译:Viorio vulnificus的螃蟹和日本泥虾及其分离物的血清分型和药物敏感性

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摘要

To clarify the route and source of Vibrio vulnificus infection, we analyzed contamination in commercially available crustaceans with V. vulnificus and conducted serotyping and drug sensitivity tests with various antimicrobial agents. We isolated V. vulnificus from 328 of 4,769 samples (6.9%) of crustaceans. V. vulnificus were most frequently isolated from 272 of 1954 mantis crabs (13.9%), followed by 22 of 434 (5.1%) Japanese mud shrimp and 34 of 2,381 (1.4%) shrimp. Regarding the isolation rates ofV. vulnificus according to region, V. vulnificus was isolated in10 of12 prefectures (83.3%) surveyed and was most frequently isolated in Miyagi (40.0%), followed by Kagoshima (20.8%), but was not found in any samples from Kagawa or Fukui, indicating a regional difference. As a result of serotyping, 208 of 328 (63.4%) examined strains were differentiated into14 serotypes : serotype 019 was the most frequently observed (13.7%), followed by 016 (10.3%), 014 (7.3%) and 023 (7.3%). The results of MIC90 in drug susceptibility tests were compared. Ciprofloxacin was the lowest, followed by Meropenem, Minocycline, Tetracyclin, Doxycycline, Chloramphenicol and Nalidixic acid and all strains were susceptible to all of these 7 drugs. However, Lincomycin, Cefmetazole, Kanamycin, Amikacin, Ampicillin, Piperacillin, Cefaloridine, Cefalotin, Cefozopran, Gentamicm, Erythromycin, Cefotaxime and Latamoxef showed high MIC_(90), and many strains were resistant to those13 drugs.
机译:为了弄清楚弧菌弧菌感染的途径和来源,我们分析了市售甲壳动物中的弧菌弧菌污染,并使用各种抗菌剂进行了血清分型和药物敏感性测试。我们从4,769例甲壳动物样本中的328例中分离出了V. vulnificus。 V. n。Vulnificus最常见的是从1954年的螳螂蟹中的272只(13.9%)中分离出来,其次是434只日本泥虾中的22只和2,381只(1.4%)虾中的34只。关于V的隔离率。根据地区划分,在12个县中有10个(83.3%)分离到了V. vulnificus,最常见的是在宫城县(40.0%),其次是鹿儿岛(20.8%),但在香川县或福井县的任何样本中都没有表示区域差异。进行血清分型,将328个菌株中的208个(63.4%)分化为14个血清型:最常见的是019型(13.7%),其次是016(10.3%),014(7.3%)和023(7.3%) )。比较了MIC90在药敏试验中的结果。环丙沙星最低,其次是美罗培南,米诺环素,四环素,强力霉素,氯霉素和萘啶酸,所有菌株均对这7种药物均敏感。但是,林可霉素,头孢美唑,卡那霉素,丁胺卡那霉素,氨苄青霉素,哌拉西林,头孢洛啶,头孢洛丁,头孢唑普兰,庆大霉素,红霉素,头孢噻肟和拉他莫昔均显示高MIC_(90),许多菌株对那13种药物有抗药性。

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