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首页> 外文期刊>日本食品微生物学会雑誌 >Two Rare Contaminants, Helicostylum pulchrum and Scopulariopsis flava, Found in a White Natural Cheese, and the Effect of Their Presence
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Two Rare Contaminants, Helicostylum pulchrum and Scopulariopsis flava, Found in a White Natural Cheese, and the Effect of Their Presence

机译：在白色天然奶酪中发现的两种稀有污染物Helicostylum pulchrum和Scopulariopsis flava及其存在的影响

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摘要

Two rare moulds, Helicostylum pulchrum and Scopulariopsis flava, were isolated from a brown stain found on the cut surface of commercially available Brie cheese. Spoilage yeast De-baryomyces hansenii was also present in the same portion. H. pulchrum is psychrophilic but not commonly found as spoilage mycobiota on natural cheese. It grew rapidly on malt extract agar or Brie cheese at 10°C, while D. hansenii and S. flava developed at a moderate and quite slow rate, respectively, under the same condition. To examine the relationship between the contaminants and a discoloration, fungal spike trials were carried out. A reddish or yellowish discoloration was found on the spiked cheese slices by the inoculum of D. hansenii alone or D. hansenii combined with H. pulchrum or/and S. flava. It was not pigments produced by spiked species, but caused by lactic acid bacteria. Concerning H. pulchrum- or D. hansenii-damaged cheeses, cytotoxic assay was done using human neuroblastoma cells. The extracts of these damaged cheeses exhibited no toxicity to the human cells.
机译：从市售Brie奶酪切面上发现的棕色污渍中分离出两种稀有的模具Helicostylum pulchrum和Scopulariopsis flava。腐败的酵母De-Baryomyces hansenii也存在于同一部分。 H. pulchrum具有嗜热性，但通常不会在天然奶酪上发现变质的真菌菌落。它在10°C的麦芽提取物琼脂或布里干酪奶酪上迅速生长，而汉森氏梭菌和弗氏链霉菌在相同条件下分别以中等和相当慢的速度生长。为了检查污染物与变色之间的关系，进行了真菌加标试验。单独接种汉氏梭状芽孢杆菌或汉氏梭状芽孢杆菌与H.pulchrum或/和S. flava的接种物，在加标的奶酪切片上发现带红色或淡黄色的变色。它不是由加标物种产生的色素，而是由乳酸菌引起的。对于H.pulchrum或hansenii损害的奶酪，使用人神经母细胞瘤细胞进行了细胞毒性测定。这些受损奶酪的提取物对人体细胞无毒性。

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