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首页> 外文期刊>日本食品微生物学会雑誌 >Applicability of the ISO Enterobacteriaceae Test for Determining the Suitability of Pasteurized Milk for shipment
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Applicability of the ISO Enterobacteriaceae Test for Determining the Suitability of Pasteurized Milk for shipment

机译：ISO肠杆菌科测试用于确定巴氏杀菌牛奶是否适合运输的适用性

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We compared the desoxycholate agar (DOA) media method to investigate the presence of coliforms at milk plants and its proposed substitute, the ISO Enterobacteriaceae test. In raw milk samples with only coliforms or Enterobacteriaceae, there was a goodcorrelation (r^0.98) between the number of bacteria on DOA and violet red bile glucose (VRBG) agar. In raw milk samples that contained Pseudomonas, which is a psychrotrophic bacteria, as well as coliforms or Enterobacteriaceae, the correlation between the number of bacteria on DOA at 30degC and VRBG agar at 37degCwas weak. It was assumed that this was because the capability of supporting the growth of psychrotrophic bacteria by VRBG agar is inferior to that demonstrated by DOA; the difference in incubation temperatures greatly influenced this effect. The ratio of the bacteria grown on VRBG/ DOA agar plates (VRBG/DOA) using the coliforms such as E. aerogenes NBRC 13534T and K. oxytoca JCM1665 was 0.67-1.09 and 0.95-1.31, respectively. Because the bacterial count of Pseudomonas grown on VRBG agar was not suitably counted, the count differed depending on the strains used, incubation temperature, and layering conditions. For tests verifying the suitability of raw milk for shipment, if the purpose is to detect the presence of Enterobacteriaceae only, then the ISO Enterobacteriaceae test can be used. However, if psychrotrophic bacteria are also to be detected, then using the ISO Enterobacteriaceae test may not be suitable.
机译：我们比较了脱氧胆酸盐琼脂（DOA）培养基方法来研究牛奶植物及其拟议替代品ISO肠杆菌科测试中大肠菌的存在。在仅含有大肠菌或肠杆菌科的原奶样品中，DOA细菌数与紫红色胆汁葡萄糖（VRBG）琼脂之间存在良好的相关性（r ^ 0.98）。在包含假单胞菌（一种精神营养细菌）以及大肠菌或肠杆菌科的原奶样品中，DOA在30degC和VRBG琼脂在37degC的细菌数量之间的相关性较弱。可以认为这是因为VRBG琼脂支持精神营养细菌生长的能力不如DOA所证明的。孵育温度的差异极大地影响了这种效果。使用大肠埃希氏菌NBRC 13534T和产氧耶尔森菌JCM1665等大肠菌在VRBG / DOA琼脂平板（VRBG / DOA）上生长的细菌比例分别为0.67-1.09和0.95-1.31。由于未对VRBG琼脂上生长的假单胞菌的细菌计数进行适当计数，因此该计数会根据所用菌株，孵育温度和分层条件而有所不同。对于检验原奶是否适合运输的测试，如果目的是仅检测肠杆菌科的存在，则可以使用ISO肠杆菌科的测试。但是，如果也要检测精神营养菌，则可能不适合使用ISO肠杆菌科测试。

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